FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_13.3510000005b2b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_13.3510000005b2b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51982
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA2190.42129968065868956No Hit
GACTCTATGGTGGCTGTCTCTTATACA1790.3443499672963718No Hit
GACTCTATGGTGCCTGTCTCTTATACA1410.27124773960216997No Hit
GACTCTATGGTGTCTGTCTCTTATACA990.19045054057173638No Hit
GACTCTATGGTGCTGTCTCTTATACAC580.1115770843753607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG2700.021.2458533
CTCTATG46400.021.2458533
GACTGTA303.571294E-421.2458531
GACTATA2700.021.2458531
ACTATAT2700.021.2458532
AATCTAT557.4232958E-921.2458522
ATCTATG557.4232958E-921.2458523
GAATCTA557.4232958E-921.2458521
TTCTATG800.021.2458523
ATTCTAT800.021.2458522
GACTCTA44950.021.222221
ACTCTAT44900.021.2221952
GTGTCTA250.003374599920.95957810
GTGTTTC250.003374599920.95957810
GGTGAGG1300.020.9595789
ACGCTTT250.003374599920.95957817
GGTGACG701.4551915E-1120.9595789
GGCATTT354.5622302E-520.95957817
AGCTTTT354.5622302E-520.95957818
GTGCATC250.003374599920.95957810
Produced by FastQC (version 0.11.2)