FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_12.3510000005b299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_12.3510000005b299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114284
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC8410.7358860382905743No Hit
GAATCCTTACGCCTGTCTCTTCTACAC2560.22400336005040075No Hit
GAATCCTTACGCTGTCTCTTATACACA1620.14175212628189424No Hit
GAATCCTTACGCCCTGTCTCTTATACA1490.1303769556543348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTA4000.021.2556613
GTATCCT2300.021.255661
TTCCTTA2450.021.255663
GAATACT3800.021.255661
ATACTTA3800.021.255663
ATTCCTT2450.021.255662
ATCCTTA101600.021.255663
AATCCTT99350.021.2342642
GAATCCT99400.021.2235831
ACTCCTT4050.020.9932442
GACTCCT4050.020.9932441
AATACTT3850.020.9796122
ACGCTTG1350.020.9579899
ACGCTGA2250.020.9579899
ACGCCCC1350.020.9579899
AGGGGTT507.5715434E-820.95798716
GGAGATT250.003400692320.95798716
TTGTCTT250.003400692320.95798716
CGCGAAT354.6270714E-520.95798710
CGTTTCT354.6270714E-520.95798719
Produced by FastQC (version 0.11.2)