FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate1_10.3510000005b27f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate1_10.3510000005b27f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53100
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC4440.8361581920903955No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1730.3258003766478343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC46300.021.223071
ACGTATC3700.021.223071
ACTTCTC850.021.223071
TTCTCTG850.021.223073
CGTATCT3700.021.223072
CTTCTCT850.021.223072
CGTCTCT46300.021.223072
ACATCTC303.5988307E-421.2230681
CGTGTCT303.5988307E-421.2230682
ACGTGTC303.5988307E-421.2230681
GTCTCTG46850.021.200423
AGGGGTT354.5584795E-520.96327816
TGCATTA2200.020.9632788
ATGGGCG405.332242E-620.96327811
GTGTTTT1600.020.96327818
CATTGTG354.5584795E-520.96327810
GCCTTTT651.2187229E-1020.96327818
CAGTTTT850.020.96327818
CATGAGG558.6783984E-920.96327810
TGCATAT2200.020.9632788
Produced by FastQC (version 0.11.2)