FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_95.3520000006122b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_95.3520000006122b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA333.2352941176470593No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA30.29411764705882354No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA30.29411764705882354No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC30.29411764705882354No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA30.29411764705882354No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC30.29411764705882354No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGGCTCTCCAGTCTGCTAACGTACTCTGCGTTG20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT20.19607843137254902No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA20.19607843137254902No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AAAAAAAAAAAAGCGTAAAATCCAGTCTGCTAA20.19607843137254902No Hit
GTGAATGGGGTCCAGTCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GCTTTCCAGTCTGCTAACGTACTCTGCGTTGAT20.19607843137254902No Hit
GACTGGAGAGTCAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AATGGGCGCCTCCAGTCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AAAAAAATGAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTCGAGCCTCCAGTCTGCTAACGTACTCTGCGT20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGTCCAGTCAAAAA20.19607843137254902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CCAGTCTGCTAACGTACTCTGCGTTGATACCAC20.19607843137254902No Hit
CTCCGGAAAGGTGCAGCGACAAAAAAAAAAAAA20.19607843137254902No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GCTCCTTCCAGTCTGCTAACGTACTCTGCGTTG20.19607843137254902No Hit
TCCCTTCCAGTCTGCTAACGTACTCTGCGTTGA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)