FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_94.35200000061201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_94.35200000061201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343.3333333333333335No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
ACGTTACTGCAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGGTACGTTACTGCAAAAA30.29411764705882354No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC30.29411764705882354No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA30.29411764705882354No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA30.29411764705882354No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGCGACCTCAAAA20.19607843137254902No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACTCTCATTCTCCTTGCAGTAACGTACTCTGCG20.19607843137254902No Hit
CTAAACCCTCCTTGCAGTAACGTACTCTGCGTT20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTTACT20.19607843137254902No Hit
CGTCCCCTCCTTGCAGTAACGTACTCTGCGTTG20.19607843137254902No Hit
CCCCCTCCTTGCAGTAACGTACTCTGCGTTGAT20.19607843137254902No Hit
CCCTTGCTCCTTGCAGTAACGTACTCTGCGTTG20.19607843137254902No Hit
AACGCAGAGTGAATGGGAGTACGTTACTGCAAA20.19607843137254902No Hit
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC20.19607843137254902No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC20.19607843137254902No Hit
CTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC20.19607843137254902No Hit
GGGCACCTCCTAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AACTCAGAGTGAATGGGGAGCGGGCCGGTTTTT20.19607843137254902No Hit
GCCACGCCCTCCTTGCAGTAACGTACTCTGCGT20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC20.19607843137254902No Hit
GTATCATCGCAGAGTGAATGGGACAACTCCTTG20.19607843137254902No Hit
CCCCACTCCTTGCAGTAACGTACTCTGCGTTGA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT401.6793283E-420.2527
TCCTTGC550.001463746714.72727310
TTGCAGT550.001463746714.72727313
CTCCTTG550.001463746714.7272739
TAACGTA550.001463746714.72727319
CAGTAAC550.001463746714.72727316
CCTTGCA550.001463746714.72727311
GCAGTAA550.001463746714.72727315
AGTAACG550.001463746714.72727317
CTTGCAG550.001463746714.72727312
TGCAGTA550.001463746714.72727314
GTAACGT550.001463746714.72727318
Produced by FastQC (version 0.11.2)