FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_9.35200000060786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_9.35200000060786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39314
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3490.8877244747418223No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1590.4044360787505723No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1240.3154092689627105No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1240.3154092689627105No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1190.30269115327873025No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC980.24927506740601313No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.23401332858523682No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA850.21620796662766445No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT830.21112072035407234No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA780.19840260467009208No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA770.195858981533296No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA730.1856844889861118No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA690.17550999643892762No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC640.16279188075494733No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA610.15516101134455917No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC470.11955028742941447No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA450.11446304115582234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.11446304115582234No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA450.11446304115582234No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGTA440.11191941801902631No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA420.10683217174543419No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA400.1017449254718421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.7314384E-426.9770724
ACGCTGA257.7314384E-426.9770721
CCGCCCT200.0085465626.9770725
AATCCTA300.00223718422.4808925
CGCCCTA300.00223718422.4808926
CGCTGAG350.00546694719.2693372
GCTGAGT350.00546694719.2693373
TGATACC853.1559466E-919.1156927
CATCTAC601.9805258E-517.9847136
GCCCTAC604.0669E-415.7366247
TCAACGC1601.8189894E-1215.17460253
ATCAACG1601.8189894E-1215.17460252
TATCAAC1700.015.0754211
TCTGCGT9950.014.83307227
ATCTACG1302.5975169E-914.5261147
CAACGCA1755.456968E-1213.8739224
TTGATAC1303.9261067E-813.54028126
ATCCTAC600.00742332713.4885356
GTACGTT600.00742332713.4885351
AACGCAG1953.092282E-1112.4509545
Produced by FastQC (version 0.11.2)