FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_83.352000000610cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_83.352000000610cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA363.5294117647058822No Hit
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC191.8627450980392157No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121.1764705882352942No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.7843137254901961No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC50.49019607843137253No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGGAGACCTTAAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGGGTGAGTCCAAAAAAA20.19607843137254902No Hit
GTAGTCCTTCTGTGACGTACTCTGCGTTGATAC20.19607843137254902No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGAAGAGTCCAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCGAGAGTCCAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGGCGCAGTCCAAAAAAA20.19607843137254902No Hit
GCCCAGTCCAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGCAGTCCAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGGCGGAGTCCTTAAAAAAAAAAA20.19607843137254902No Hit
CCCCGAGTCCTTCTGTGACGTACTCTGCGTTGA20.19607843137254902No Hit
GCGTTGATACCACAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGGCCAAGTCCTTCTGTGACGTACTCTGCGTTG20.19607843137254902No Hit
GAGTACGTCACAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGCAGTCCTAAAAA20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGGATCAGTCCAAAA20.19607843137254902No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)