FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_8.3520000006076c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_8.3520000006076c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30312
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA7362.42808128793877No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA3020.996305093692267No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC2550.8412509897070467No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2070.6828978622327792No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA1780.5872261810504091No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1660.5476378991818422No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1580.5212457112694642No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.4651623119556611No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA1270.41897598310899975No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA1210.39918184217471625No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1120.36949063077329114No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1090.35959356030614936No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA940.31010820797044075No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA870.28701504354711005No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC860.2837160200580628No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA850.2804169965690156No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC840.2771179730799683No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA750.24742676167854313No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA730.24082871470044864No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC710.23423066772235418No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA710.23423066772235418No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC670.2210345737661652No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA640.2111375032990235No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA600.1979414093428345No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA550.1814462918975983No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA530.17484824491950385No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA490.16165215096331484No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA480.1583531274742676No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG480.1583531274742676No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA480.1583531274742676No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA480.1583531274742676No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA460.15175508049617312No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA450.14845605700712589No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA430.1418580100290314No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA420.13855898653998416No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA410.13525996305093693No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA410.13525996305093693No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA400.13196093956188967No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA400.13196093956188967No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC400.13196093956188967No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA400.13196093956188967No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA380.1253628925837952No Hit
CCCCCCATGAGCACTGTACGTACTCTGCGTTGA370.12206386909474796No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA370.12206386909474796No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC370.12206386909474796No Hit
CTGTACGTACTCTGCAAAAAAAAAAAAAAAAAA350.11546582211665347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.11546582211665347No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA330.108867775138559No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT330.108867775138559No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA320.10556875164951175No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA320.10556875164951175No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA310.10226972816046451No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT310.10226972816046451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.008498386526.988411
GTATCAT356.3295192E-626.988411
AACTCAG200.008498386526.988412
CTCAGAG200.008498386526.988414
ATCATCG356.3295192E-626.988413
GAGAATG200.0085674626.94380413
TCGCCAT200.0085674626.9438045
TCAGAGT200.0085674626.9438045
AGAGAAT200.0085674626.94380412
CAGAGAG257.745751E-426.9438029
GCAGAGA257.745751E-426.9438028
ATCGCAG356.411896E-626.9438026
AGAGAGA307.0336326E-526.943810
CGCCATG451.6379126E-623.9500476
TCTACGC401.7833216E-523.614863
TATCATC401.7833216E-523.614862
CTACGCA401.7833216E-523.614864
TCGCAGA401.806433E-523.5758297
ATCTACG351.9816053E-423.1329232
TATCTAC351.9816053E-423.1329231
Produced by FastQC (version 0.11.2)