FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_79.35200000061054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_79.35200000061054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282.7450980392156863No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA50.49019607843137253No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA30.29411764705882354No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA30.29411764705882354No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGTACGTCAGACATCACAAAAAAAAA30.29411764705882354No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
TGCGATGTCTGACGTACTCTGCGTTGATACCAC20.19607843137254902No Hit
GAGTGAATGGGATGTCTGACGTACTCTAAAAAA20.19607843137254902No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGATTGCGATGTCT20.19607843137254902No Hit
GAATGGGGAGTACGTCAGACATCGCAAAAAAAA20.19607843137254902No Hit
GACTAAAATTGCGATGTCTGACGTACTCTGCGT20.19607843137254902No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA20.19607843137254902No Hit
ATCAACGCAGAGTGAATGGGATGTCTGACGTAC20.19607843137254902No Hit
GTGAATGGGTTGCGATGAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGTCAGACATCACAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGTTGCGATGTAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGTTGCGAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ATGGGTACTCTGGTTGAACCATGCTAAAAAAAA20.19607843137254902No Hit
GTGAATGGGATTGCGATGAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA20.19607843137254902No Hit
ATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGATGTCTGACGTACTCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGATGTCTGACGTACTCAAAAAAAAAAA20.19607843137254902No Hit
ATGTCTGACGTACAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGAGACATCGCAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGGACATCGCAAAAAAAA20.19607843137254902No Hit
GTCTGACAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGTTGCGATGAAAAAAAAA20.19607843137254902No Hit
GAATGGGATTGCGATGTCAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACGTCAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TGTCTGACAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTCTTATTTGCGATGTCTGACGTACTCTGCGTT20.19607843137254902No Hit
CAACGCAGAGTGAATGGGACATCGCAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGACCATTGCGATGAAAAAAAAAA20.19607843137254902No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC20.19607843137254902No Hit
AAATGACTCCATTGCGATGTCTGACGTACTCTG20.19607843137254902No Hit
TCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)