FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_70.35200000060f21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_70.35200000060f21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA363.5294117647058822No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC90.8823529411764706No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC90.8823529411764706No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA80.7843137254901961No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA60.5882352941176471No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA60.5882352941176471No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA50.49019607843137253No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG50.49019607843137253No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA40.39215686274509803No Hit
GTAGTTCTGACACGTACTCTGCGTTGATACCAC40.39215686274509803No Hit
CAACGCAGAGTGAATGGGGTAGTTCAAAAAAAA30.29411764705882354No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA30.29411764705882354No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC30.29411764705882354No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GAGTGAATGGGGTAGTTCTGACAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA30.29411764705882354No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA30.29411764705882354No Hit
GAGTGAATGGGTAGTTCTGACACGAAAAAAAAA30.29411764705882354No Hit
GTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGGTAGTTCAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC20.19607843137254902No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ATCTTACCTTACGAAATACTGAGACACAGATTG20.19607843137254902No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACGTA20.19607843137254902No Hit
TTCTGACACGTACTCAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGTAGTTCTGACAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGGTAGAAAAAAAAAAAAA20.19607843137254902No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA20.19607843137254902No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA20.19607843137254902No Hit
GTGAATGGGTAGTTCTGACCGAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGGTAGTTCAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGAGTTCTGACACA20.19607843137254902No Hit
GTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA20.19607843137254902No Hit
TCAACGCAGAGTGAATGGGTAGTTCTGACACGT20.19607843137254902No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGTAGTTCTGACACGAAAAAAAAAAAAA20.19607843137254902No Hit
AGAGAATAGAGGAGAAAACGCAAAAAGAATACA20.19607843137254902No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA20.19607843137254902No Hit
TCAACGCAGAGTGAATGGGTAGTTCTGACACTA20.19607843137254902No Hit
AACGCAGAGTGAATGGGTAGTTCAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGGTAGTTCTGACACGTACTAAAAAA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)