FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_7.35200000060750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_7.35200000060750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36734
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA2440.6642347688789677No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA2240.609789296020036No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA2070.5635106440899439No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA1520.41378559372788154No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA1150.3130614689388577No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA1120.304894648010018No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA960.26133826972287255No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA800.2177818914357271No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC790.21505961779278052No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG730.198725975935101No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA730.198725975935101No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA700.19055915500626122No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA550.1497250503620624No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA490.13339140850438286No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA490.13339140850438286No Hit
TATCTACGCAGAGTGAATGGGCATTGCTGAAGA490.13339140850438286No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA480.13066913486143628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1279468612184897No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA470.1279468612184897No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA460.1252245875755431No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG450.1225023139325965No Hit
GTCTTCAGCAATGCAAAAAAAAAAAAAAAAAAA450.1225023139325965No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA440.1197800402896499No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG440.1197800402896499No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.11433549300375674No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA410.11161321936081015No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA400.10889094571786355No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA400.10889094571786355No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA400.10889094571786355No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA390.10616867207491695No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA370.10072412478902379No Hit
CCCCCGCATTGCTGAAGACGTACTCTGCGTTGA370.10072412478902379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT257.710774E-426.9836526
CAGGCAT300.002231257722.4863766
AGGCATT658.001007E-820.7566557
GGCGCAT350.00545259519.2740366
TACGCAT501.0051392E-418.8885566
GCGTTGA2250.018.03825227
ATCAACG2800.017.3466322
TATCAAC3050.017.2518421
GCGCATT1309.094947E-1216.6053247
ACGCATT1258.367351E-1116.1901917
TCAACGC3050.015.9247793
CAACGCA3350.014.9014214
TCTGCGT10150.014.52834527
AACGCAG3350.014.0959385
CTGCATT1051.1976084E-512.8493587
GGTATCA956.774164E-512.781731
CTCTGCG12250.012.03777226
TGCATTG2850.011.8349358
GTACAAA800.003553849911.805346510
ACGCAGA4150.011.70375256
Produced by FastQC (version 0.11.2)