FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_49.35200000060c81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_49.35200000060c81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA252.314814814814815No Hit
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA211.9444444444444444No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA171.574074074074074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161.4814814814814816No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA151.3888888888888888No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA141.2962962962962963No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA131.2037037037037037No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA131.2037037037037037No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131.2037037037037037No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA121.1111111111111112No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA121.1111111111111112No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA100.9259259259259258No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA80.7407407407407408No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA80.7407407407407408No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT80.7407407407407408No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT70.6481481481481481No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC70.6481481481481481No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA70.6481481481481481No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA70.6481481481481481No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA60.5555555555555556No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA60.5555555555555556No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA60.5555555555555556No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA50.4629629629629629No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA50.4629629629629629No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA50.4629629629629629No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCT50.4629629629629629No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA40.3703703703703704No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA40.3703703703703704No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3703703703703704No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA40.3703703703703704No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA30.2777777777777778No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA30.2777777777777778No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA30.2777777777777778No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA30.2777777777777778No Hit
CAACGCAGAGTGAATGGGCAGACAAAAAAAAAA30.2777777777777778No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CACACGAACTCAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGTAGAGTGAATGGGCAGACACAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA20.1851851851851852No Hit
GTGAATGGGCAGACACACGTACTCTAAAAAAAA20.1851851851851852No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.1851851851851852No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CGCAGATTGAATGGACAGACACAAAAAAAAAAA20.1851851851851852No Hit
CAACGCAGAGTGAATGGGCAAAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGCAGTGTGAATGGGCAGACACAAAAA20.1851851851851852No Hit
GAGTAAATGGGCAGACAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CAACGCAGAGTGAATGGGCAGACACACGTAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA20.1851851851851852No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA20.1851851851851852No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CAGAGTGAATGGGCAGACAAAAAAAAAAAAAAA20.1851851851851852No Hit
AACGTAGAGTGAATGGGCAGACAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGCAGACACACGTACAAAAAAAAAAA20.1851851851851852No Hit
GAAGAATACATTGTGTAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA20.1851851851851852No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA20.1851851851851852No Hit
GAGTGAATGGGCAGACACACGTACAAAAAAAAA20.1851851851851852No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CGTCCCTTATGAGCAGACACACGTACTCTGCGT20.1851851851851852No Hit
GAGTGAATGGGCAGACACACGTACTCTAAAAAA20.1851851851851852No Hit
ATCATCGCAGAGTGAATGGGCAGACACAAAAAA20.1851851851851852No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA20.1851851851851852No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA20.1851851851851852No Hit
AAGATGAGCAGACACACGTACTCTGCGTTGATA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGCAAAAAAAAAAA20.1851851851851852No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG20.1851851851851852No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA20.1851851851851852No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTAA20.1851851851851852No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA20.1851851851851852No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTATCAATGCAGAGTGAATGGGCAGACACACGT20.1851851851851852No Hit
CAGAGTGAATGGGCAGACACAAAAAAAAAAAAA20.1851851851851852No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)