FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_48.35200000060c74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_48.35200000060c74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18007
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA3291.8270672516243682No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA740.41095129671794306No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.33320375409562947No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA490.27211639917809743No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA460.25545621147331593No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA440.2443494196701283No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA440.2443494196701283No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA420.23324262786694064No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.21658244016215916No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA330.1832620647525962No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA320.1777086688510024No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA310.17215527294940858No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA310.17215527294940858No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA290.16104848114622092No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC270.14994168934303326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.14438829344143944No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA260.14438829344143944No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA250.13883489753984563No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA250.13883489753984563No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA250.13883489753984563No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA240.1332815016382518No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA220.12217470983506415No Hit
CCCCAGTTCCACGCTTAACGTACTCTGCGTTGA220.12217470983506415No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA210.11662131393347032No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA210.11662131393347032No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA200.11106791803187649No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA200.11106791803187649No Hit
GTTCCACGCTTAACGTACTCTTCGTTGATACCA200.11106791803187649No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA200.11106791803187649No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG190.10551452213028267No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA190.10551452213028267No Hit
CCCCCGTTCCACGCTTAACGTACTCTGCGTTGA190.10551452213028267No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA190.10551452213028267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA603.1686795E-822.49999827
CGTTGAT553.4990626E-722.0909123
GTTGATA608.084462E-720.24999824
TGATACC608.084462E-720.24999826
TTGATAC608.084462E-720.24999825
GCGTTGA1600.018.562527
ATGGGAG450.001053616418.0000025
ATGGGGA851.2401924E-615.8823545
AATGGGA1406.339178E-913.54
TATCAAC1051.623621E-411.5714291
TGCGTTG2700.011.026
TGAATGG4450.010.9213492
GTGAATG4550.010.6813181
GTATCAA900.008227088510.5000011
ATCAACG1205.5968226E-410.1249992
CTGCGTT3151.8189894E-129.85714325
AATGGGG2202.9209332E-89.8181824
CAACGCA1258.1485615E-49.7199994
TCTGCGT4900.09.64285827
GAATGGG4800.09.5624993
Produced by FastQC (version 0.11.2)