FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_47.35200000060c4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_47.35200000060c4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46578
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA1180.25333848598050585No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1120.24045686804929364No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC900.1932242689681824No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT870.18678346000257634No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA750.16102022414015202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.14599166988707113No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA610.1309631156339903No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA610.1309631156339903No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA570.12237537034651552No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA550.11808149770277815No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC530.11378762505904075No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC520.11164068873717205No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC470.10090600712782857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC350.005404814619.31650427
GCCTTCC350.00547266819.2749615
GCATCCA350.00547266819.2749616
CTATCCA450.001092684517.9899646
TTGATAC901.3142562E-716.5263427
GTTGATA952.431443E-715.65653326
GCCTCCA801.3565592E-515.1790316
CCTTCCA801.3565592E-515.1790316
CTTCCAG1950.014.5303557
TGCGTTG4550.014.26449427
AATCCAG1354.487447E-913.9921957
TATCAAC1458.058123E-1013.9577322
TCTGCGT15950.013.90303827
CGTTGAT1203.3920915E-612.39475625
CACTCCA1001.10639194E-412.1432256
CTCTCCA906.275801E-411.9933096
TATCCAG1708.6656655E-911.9051237
AATGGGT800.003577264511.8059134
TCTCCAG1852.390152E-911.6691667
CTCTGCG19600.011.38293926
Produced by FastQC (version 0.11.2)