FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_46.35200000060c24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_46.35200000060c24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88291
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC3730.4224666160763838No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA2430.2752262405001642No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA2260.25597172984788935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.15969917658651506No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1100.12458801010295499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCT200.00859092626.9852522
ATCTACG200.00859092626.9852522
AGTAAAT200.00859092626.9852522
GTATCAT356.519558E-626.985251
TCTACGC200.00861474426.9699543
TACGCAG200.00861474426.9699545
GATTCTC200.00861474426.9699546
CTACGCA200.00861474426.9699544
CTTTCAG401.8444423E-523.5987113
TCCTTTC401.8444423E-523.5987111
TCAGTAA401.8444423E-523.5987116
CCTTTCA401.8444423E-523.5987112
ACGTACG300.002247903722.50047321
TATCTAC300.002255337322.487711
GAGTAAA300.002255337322.487711
TCTGCCT300.002262791222.4749624
ACGACCT300.002262791222.4749624
GTAACTT504.1856547E-621.58820217
AGTAACT504.2063257E-621.57596416
TATCATC454.5601977E-520.988532
Produced by FastQC (version 0.11.2)