FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_42.35200000060bac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_42.35200000060bac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29712
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2090.7034194938072159No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA1980.6663974151857834No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC1250.42070543887991385No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA1130.3803177167474421No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA1070.36012385568120625No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG910.30627355950457724No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA900.30290791599353795No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA870.29281098546042No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA660.22213247172859452No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG630.21203554119547657No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA590.19857296715131934No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA580.19520732364028No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA530.17837910608508345No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA520.17501346257404415No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA510.17164781906300483No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA490.1649165320409262No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA460.15481960150780827No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA450.15145395799676897No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT410.13799138395261173No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA390.13126009693053312No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.13126009693053312No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA390.13126009693053312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1245288099084545No Hit
GTGAATGGGAAACGTACTCTGCGTAAAAAAAAA360.12116316639741519No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.11779752288637588No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA350.11779752288637588No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA350.11779752288637588No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG340.11443187937533657No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA340.11443187937533657No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA330.11106623586429726No Hit
GAAACGTACTCTGCGTTAAAAAAAAAAAAAAAA330.11106623586429726No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA310.10433494884221862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT257.6654484E-426.98994
TAGAGTG257.6654484E-426.98995
GTGCAGT200.00849386726.9898996
CTACGCA200.00849386726.9898994
AACGTAG300.00221828922.4915811
ACGTAGA300.00221828922.4915812
CGTAGAG300.00221828922.4915813
TCAGAGT404.883104E-420.2424245
ACTCAGA450.00107871117.9932653
CTCAGAG450.00107871117.9932654
GTATCAA1052.3628672E-916.7080331
CAACTCA550.00412343514.7217641
TATCAAC1651.8189894E-1214.7217621
TGCAGTC954.1812273E-614.205217
ATCAACG1552.048182E-913.05962852
ATCAGTC750.00216958812.5952867
TCCAGTC1203.349509E-612.370377
TCTGCGT11200.012.19007527
CAACGCA1852.219167E-911.6713084
TCAACGC1751.247281E-811.56713
Produced by FastQC (version 0.11.2)