FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_41.35200000060b9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_41.35200000060b9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42135
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA3050.7238637712115819No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1050.2491990032039872No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT850.20173252640322772No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA660.15663937344250622No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC620.14714607808235433No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.13053281120208854No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA520.1234128396819746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.12103951584193663No Hit
GAGTACGTTCAACCAAAAAAAAAAAAAAAAAAA470.11154622048178474No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA460.10917289664174677No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.10442624896167083No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA430.10205292512163285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.9985115E-527.0000021
ATCATCG306.9985115E-527.0000023
TCATCGC306.9985115E-527.0000024
CATCGCA306.9985115E-527.0000025
GTACCTA257.7023794E-427.06
GATACCA200.00851802627.027
TCGCAGA200.00851802627.07
ATCGCAG200.00851802627.06
TATCATC351.9939756E-423.1428592
TCTGCGT12450.021.57831427
AACCCTA404.9176393E-420.256
TACCCTA501.0049283E-418.9000026
AATCCTA601.9715653E-518.0000026
AGCCTAT1002.4638211E-816.27
CTCTGCG17550.015.38461526
TGCCTAT1158.179995E-915.2608697
TCCCTAT1601.8189894E-1215.18757
TATCAAC1454.9112714E-1114.8965521
ATCCCTA550.004151422514.7272736
ATCCTAT1701.8189894E-1214.2941187
Produced by FastQC (version 0.11.2)