FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_40.35200000060b75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_40.35200000060b75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37574
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2440.6493852131793262No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA2020.5376057912386225No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1030.27412572523553524No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC630.1676691329110555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.15436205887049556No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA540.14371639963804758No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT540.14371639963804758No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA480.12774791078937564No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA470.12508649598126365No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA460.12242508117315165No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC450.11976366636503966No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA440.11710225155692766No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA420.11177942194070369No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA410.10911800713259169No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA390.1037951775163677No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA380.1011337627082557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC200.00850863226.9986698
CGTACCT200.00850863226.9986699
GATACCA601.96322E-517.99911127
TGATACC653.9046077E-516.61456526
TAGTCAA500.002194354316.1992027
TATCAAC1252.3874236E-814.0580261
TCTGCGT4900.014.05032727
GTATCAA802.267325E-413.5173341
GTTGATA802.2932055E-413.49933424
TTGATAC802.2932055E-413.49933425
CGTTGAT802.2932055E-413.49933423
TGCGTTG1851.2732926E-1113.13448727
AGTCAAG1401.0729855E-712.5350968
CTCTGCG5850.011.7686526
TCAACGC1502.6345515E-711.6994223
ATCAACG1502.6345515E-711.6994222
TGTCAAG1751.2871169E-811.5708598
ACTCTGC6300.010.92803325
CAACGCA1659.0399226E-710.63583854
CTGCGTT2305.529728E-1010.56469626
Produced by FastQC (version 0.11.2)