FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_37.35200000060b18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_37.35200000060b18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29711
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA3371.1342600383696273No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA1610.5418868432567062No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA1570.528423816095049No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1560.5250580593046347No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA1240.41735384201137626No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.37023324694557574No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA850.2860893271852176No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA630.21204267779610245No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA620.20867692100568813No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA610.2053111642152738No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC530.17838510989195921No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA510.17165359631113056No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT450.1514590555686446No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA450.1514590555686446No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA390.13126451482615867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11106997408367272No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA300.10097270371242974No Hit
CTCGGTACTAGAGACGTACTCTGCGTTGATACC300.10097270371242974No Hit
TATCAACGCAGAGTGAATGGGTCGGTACTAGAG300.10097270371242974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA1350.020.02
CTAGTAC1500.018.93
CTCTAGT1400.018.3214281
TAGTACC1500.018.04
AGTACCG1500.017.0999985
TCTGCGT3950.016.74683627
TATCAAC1600.016.031251
ATCAACG1650.015.5454552
TACCGAA707.2758456E-515.4285727
TCAACGC1700.015.0882343
GTACCGA1651.8189894E-1214.7272736
ACCGAAA550.004113187514.7272738
GTATCAA954.165033E-614.2105261
CAACGCA1850.013.8648644
CATCGGT600.007325971513.57
CCGAAAA600.007325971513.59
CTCTGCG5450.012.13761526
ACTCGGT850.005520434611.1176467
AACGCAG2202.419256E-1011.0454555
ACTCTGC6300.010.525
Produced by FastQC (version 0.11.2)