FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_35.35200000060ad3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_35.35200000060ad3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54558
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC6141.1254078228674071No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.4728912350159463No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.26027347043513327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.2272810586898347No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1080.1979544704717915No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA840.15396458814472672No Hit
CCCCAGTCCTTCTGTGACGTACTCTGCGTTGAT710.13013673521756663No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA670.12280508816305584No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.11730635287217274No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA640.11730635287217274No Hit
CCCCTAGTCCTTCTGTGACGTACTCTGCGTTGA640.11730635287217274No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC570.10447597052677883No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA560.10264305876315115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA604.1836756E-1127.05703726
GTATCAT405.936745E-726.9825691
TATCATC405.936745E-726.9825692
TGATACC651.1459633E-1024.97572725
TCGCAGA451.6580289E-623.9845077
CATCGCA451.6580289E-623.9845075
ATCATCG401.8210832E-523.6097473
TCATCGC401.8210832E-523.6097474
ATACCAC702.8740033E-1023.213127
GTATCTA352.0133771E-423.1279161
ACTCAGA352.0133771E-423.1279163
TTGATAC756.87578E-1021.6456324
TCAGAGT504.140815E-621.5860545
CTCAGAG454.522667E-520.9864434
GTTGATA801.538865E-920.29277823
ATCGCAG559.447149E-619.6236886
GCGTTGA5250.018.3125627
CAACTCA552.1100981E-417.1707271
ATCTACG500.002220424816.189542
TCTACGC500.002220424816.189543
Produced by FastQC (version 0.11.2)