FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_33.35200000060a90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_33.35200000060a90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19927
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1010.5068500025091585No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA960.4817584182265269No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT910.4566668339438952No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA730.3663371305264214No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA680.34124554624378983No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA560.281025743965474No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT560.281025743965474No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC560.281025743965474No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA560.281025743965474No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC550.27600742710894766No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA540.27098911025242134No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA540.27098911025242134No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.2609524765393687No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA500.25091584282631607No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA450.22582425854368446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1957143574045265No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA370.18567772369147387No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA360.18065940683494755No Hit
TAGCCATTCCGTTACGTACTCTGCGTTGATACC340.17062277312189492No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT330.1656044562653686No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.13549455512621067No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA260.13047623826968435No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA260.13047623826968435No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA260.13047623826968435No Hit
AAGCCATTCCGTTACGTACTCTGCGTTGATACC240.12043960455663172No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA230.11542128770010537No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA220.11040297084357906No Hit
CCCCTAGCCATTCCGTTACGTACTCTGCGTTGA220.11040297084357906No Hit
GAGTACGTAAAAAAAAAAAAAAAAAAAAAAAAA210.10538465398705274No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA200.10036633713052642No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTAACG200.10036633713052642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTA200.00841217127.013
CTTTACG200.00841217127.015
CATTCCT200.00841217127.010
TTCCTTT200.00841217127.012
CCTTTAC200.00841217127.014
ATTCCTT200.00841217127.011
TTTACGT200.00841217127.016
GCGTTGA2500.019.4427
TGATACC500.002145028716.227
GTTGATA600.00721409413.50000125
TTGATAC600.00721409413.50000126
TAAGCCA700.001260592613.55
TGCGTTG4800.013.21875127
TATCAAC1305.5697274E-712.4615381
CTGCGTT5950.010.89075626
TCAACGC1452.611539E-510.241383
ATCAACG1452.611539E-510.241382
AAGCCAT2051.2849396E-69.2195126
AATGGGA1504.235874E-49.04
CAACGCA1651.0787273E-49.04
Produced by FastQC (version 0.11.2)