FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_31.35200000060a5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_31.35200000060a5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39252
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1360.3464791602975645No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1220.3108121879139916No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA930.23693060226230508No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA770.19616834810965048No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA760.19362070722510955No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA750.19107306634056864No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA750.19107306634056864No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA700.17833486191786405No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA570.14521553041883214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.13757260776520944No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA530.1350249668806685No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA510.12992968511158667No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT470.11973912157342301No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.11464383980434119No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA450.11464383980434119No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA430.10954855803525934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGA257.7314384E-426.9770727
ATCATCG257.7314384E-426.9770723
GGGGTCA200.0085465626.9770727
ATCGCAG257.7314384E-426.9770726
TATCATC300.00223718422.4808922
GTATCAT350.00546694719.2693371
TCATCGC350.00546694719.2693374
TGATACC550.004063172714.771214527
GGGGACA550.004168324614.7147667
TCTGCGT5600.013.78207327
GTTGATA903.9555256E-513.54028127
CGTTGAT956.57185E-512.82763626
TGCGTTG2201.8189894E-1212.30934527
GTATCAA1401.4997913E-611.5616021
TATCAAC1554.091089E-711.3129651
ACTTGCG1154.0327845E-410.5562458
ATCAACG1701.347029E-610.3147622
GCGTTGA1205.7634665E-410.155210525
TCAACGC1751.954013E-610.0200553
ATGGGGA1351.5666228E-49.9915085
Produced by FastQC (version 0.11.2)