FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_30.35200000060a33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_30.35200000060a33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14644
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA1300.8877355913684786No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.47801147227533464No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA580.3960666484567058No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA550.3755804425020486No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA490.33460803059273425No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA480.32777929527451516No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA450.30729308931985794No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA440.3004643540016389No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA410.2799781480469817No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA400.27314941272876264No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA350.23900573613766732No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA330.22534826550122916No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA330.22534826550122916No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA310.21169079486479106No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA310.21169079486479106No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA270.18437585359191477No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA270.18437585359191477No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA250.17071838295547664No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA220.15023217700081945No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA210.14340344168260039No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC200.13657470636438132No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA200.13657470636438132No Hit
GTGAATGGGTTCGTCACACTACGTACAAAAAAA190.12974597104616223No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA190.12974597104616223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12291723572794319No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA170.11608850040972411No Hit
GTATCAACGCAGAGTGAATGGGTTCGTCACACT160.10925976509150505No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA160.10925976509150505No Hit
GAGTGAATGGGTTCGTCAAAAAAAAAAAAAAAA160.10925976509150505No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA160.10925976509150505No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA150.102431029773286No Hit
GTACGTAGTGTGACAAAAAAAAAAAAAAAAAAA150.102431029773286No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.102431029773286No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA150.102431029773286No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT150.102431029773286No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCAAAAA150.102431029773286No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA150.102431029773286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGT1250.020.5043838
GTGTTCG558.700754E-619.621427
TCTGCGT3300.019.27866227
TTGTTCG509.542604E-518.8856167
TCAACGC509.542604E-518.8856163
ATCAACG509.542604E-518.8856162
TATCAAC601.8435238E-517.9863011
CAACGCA551.9812322E-417.1687414
TCGTTCG500.00211880216.187677
TGCGTTG550.00390967114.76663527
ATGGGTT600.0071283213.4897265
AATGGGT600.0071283213.4897264
TGGGTTC600.0071283213.4897266
CTCTGCG4850.013.39653626
ACTCTGC5100.013.00525625
GCAGAGT853.6064614E-412.6962128
AACGCAG750.002084853112.5904115
GTGAATG2600.012.4520541
TGAATGG2500.012.4105472
AATGGGG1101.7529199E-512.2633874
Produced by FastQC (version 0.11.2)