FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_29.35200000060a0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_29.35200000060a0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51978
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGGACCTTACGTACTCTGCGTTGATACCAC5080.9773365654700066No Hit
TGCTTGGACCTTACGTACTCTGCGTTGATACCA4940.9504020931932742No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA3000.5771672630728385No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA2380.4578860287044519No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA1710.3289853399515179No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1530.2943553041671476No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1410.2712686136442341No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1340.2578013775058679No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1260.24241025049059217No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA960.1846935241833083No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA840.1616068336603948No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA830.15968294278348533No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA830.15968294278348533No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT790.15198737927584746No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.15006348839893802No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA760.1462157066451191No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA760.1462157066451191No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA750.14429181576820962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12890068875293395No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA620.11928123436838663No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA620.11928123436838663No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA620.11928123436838663No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA590.11350956173765823No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA570.10966177998383933No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA530.10196621647620148No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT520.100042325599292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCAG257.5605063E-427.1005824
GTTTGGG200.00858239726.97016317
GGGTTGT200.00858239726.97016312
GTATCAT200.00858239726.9701631
CGGGTTG200.00858239726.97016311
TTGGGAA200.00858239726.97016319
TATCATC200.00858239726.9701632
TGGGAAT200.00858239726.97016320
GTCGGGT257.7771477E-426.9701639
TCGGGTT200.00858239726.97016310
GAATGCA300.002187911722.58381723
GCAGCCC300.002187911722.58381727
ATCTACG300.00225026322.4751362
TCTACGC300.00225026322.4751363
TACGCAG300.00225026322.4751365
TATCTAC300.00225026322.4751361
AGTCGGG300.00225026322.4751368
CTACGCA300.00225026322.4751364
CGTTGCT404.9761846E-420.2276216
ATGCAGC350.00534700919.35755725
Produced by FastQC (version 0.11.2)