FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_28.352000000609fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_28.352000000609fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26528
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA2290.8632388419782872No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1460.5503618817852834No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT800.30156815440289503No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA660.24879372738238842No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA630.23748492159227982No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA620.23371531966224368No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC590.22240651387213511No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA530.19978890229191798No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA470.17717129071170085No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA430.1620928829915561No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.1545536791314837No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA390.14701447527141134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.14324487334137515No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA380.14324487334137515No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA370.13947527141133897No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA360.13570566948130278No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA360.13570566948130278No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.13193606755126658No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA330.12439686369119421No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA320.12062726176115801No Hit
GCCCCATCTCGTCCTCTACGTACTCTGCGTTGA270.10177925211097708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA454.2889267E-521.06198727
TGATACC509.647533E-518.95578826
GCGTTGA1850.017.56520827
GTTGATA552.0024908E-417.23253424
TTGATAC552.0024908E-417.23253425
CACATCT550.00412023514.7148996
CGTTGAT657.145238E-414.58137523
GTATCTC700.001288302613.4886567
TGCGTTG3400.012.34515627
TATCAAC1351.2374057E-510.9907581
TCTGCGT7150.010.60463627
CTGCGTT4300.010.39104726
TATCTCG2401.0822987E-910.1164948
ATCAACG1651.0304411E-59.8099342
ACATCTC1100.00327640429.8099337
CTCTGCG8000.09.47789426
TCAACGC1752.0710122E-59.2493663
ACTCTGC9000.08.42479525
TACTCTG9450.08.02361424
AATCTCG1853.8907156E-48.0202836
Produced by FastQC (version 0.11.2)