FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_27.352000000609c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_27.352000000609c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17227
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA640.37150983920589775No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.31926626806756836No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA540.31346142682997624No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA540.31346142682997624No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA430.24960817321646253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.24380333197887039No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA340.19736460207813314No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA330.191559760840541No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA300.17414523712776456No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA290.1683403958901724No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA280.16253555465258024No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA280.16253555465258024No Hit
GAGTGAATGGGATCGTTGGAAAAAAAAAAAAAA260.15092587217739595No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA250.14512103093980377No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA240.13931618970221166No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC230.13351134846461948No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12770650722702734No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA200.11609682475184303No Hit
ACGCAGAGTGAATGGGTCGTTGGTCAAAAAAAA190.11029198351425089No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT180.10448714227665873No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA180.10448714227665873No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA180.10448714227665873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCGT200.00838453826.9970844
TATCTAC300.002140070322.5633531
ATCTACG300.002176967422.497572
TCTACGC300.002176967422.497573
CTACGCA300.002176967422.497574
TATCAAC1550.020.9620821
TACGCAG350.00532151519.283635
TGCGTTG1200.019.12293427
ATCAACG1850.016.7819712
GTTCGTT500.002130170416.1982527
TCAACGC2000.015.5233243
CAACGCA2000.015.5233244
TCTGCGT4850.015.30762527
GTATCAA802.1276863E-413.5380121
CTCTGCG5750.012.91164926
CGCAGAG2750.012.7622597
ACGCAGA2850.012.314466
ACTCTGC6200.012.19223125
AACGCAG2350.012.0625275
CAGAGTG2800.012.0522699
Produced by FastQC (version 0.11.2)