FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_26.352000000609ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_26.352000000609ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29173
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA5791.9847118911322112No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4811.6487848352929078No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT2870.983786377815103No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA2690.922085490007884No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA2600.8912350461042745No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA2380.8158228498954513No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA2010.6889932471806123No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1960.671854111678607No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.6615706303774038No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA1720.5895862612689815No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA1470.5038905837589552No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA1460.5004627566585542No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA1400.4798957940561478No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA1320.45247317725293934No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1270.4353340417509341No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA1170.40105577074692356No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA1110.3804888081445172No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1030.35306619134130873No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA1020.3496383642409077No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1020.3496383642409077No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.3427827100401056No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA930.3187879203372982No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA870.2982209577348919No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA850.29136530353408974No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA830.28450964933328765No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA820.2810818222328866No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA770.26394268673088134No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA720.24680355122887598No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA710.24337572412847494No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA680.2330922428272718No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA670.2296644157268707No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA660.22623658862646967No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA630.21595310732526654No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.21595310732526654No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA610.2090974531244644No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA600.20566962602406336No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.19881397182326122No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.19881397182326122No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA570.19538614472286017No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT570.19538614472286017No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA560.19195831762245913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1885304905220581No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1885304905220581No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA550.1885304905220581No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA530.18167483632125597No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA530.18167483632125597No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA530.18167483632125597No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA520.1782470092208549No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA510.17481918212045386No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA510.17481918212045386No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA490.16796352791965175No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA490.16796352791965175No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA480.16453570081925067No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA450.1542522195180475No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1542522195180475No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.14396873821684433No Hit
CAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA420.14396873821684433No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA420.14396873821684433No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC420.14396873821684433No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA420.14396873821684433No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA420.14396873821684433No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA410.1405409111164433No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT400.13711308401604222No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA400.13711308401604222No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA400.13711308401604222No Hit
CCCCGACACAGACAGTACGTACTCTGCGTTGAT380.1302574298152401No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA370.12682960271483906No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA370.12682960271483906No Hit
GACACAGACAGTACGTACAAAAAAAAAAAAAAA360.12340177561443799No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC360.12340177561443799No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA360.12340177561443799No Hit
AACGCAGAGTGAATGGGACACAGACAGTACGTA360.12340177561443799No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA360.12340177561443799No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA350.11997394851403695No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA350.11997394851403695No Hit
GTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.11997394851403695No Hit
GTGAATGGGACACAGACAGTACGTACTCAAAAA340.1165461214136359No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA340.1165461214136359No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA340.1165461214136359No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC330.11311829431323484No Hit
GACAGTACGAAAAAAAAAAAAAAAAAAAAAAAA320.10969046721283379No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC320.10969046721283379No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA320.10969046721283379No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC310.10626264011243272No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGTAC310.10626264011243272No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA310.10626264011243272No Hit
AATGGGACACAGACAGAAAAAAAAAAAAAAAAA310.10626264011243272No Hit
GTGAATGGGACACAGACAGTACGAAAAAAAAAA300.10283481301203168No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG300.10283481301203168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT405.681686E-727.0343631
CCACTGC257.58834E-427.03436326
TATCTAC306.865873E-527.0343631
ATCTACG306.9470014E-526.9879932
TCTACGC306.9470014E-526.9879933
TACGCAG306.9470014E-526.9879935
CTACGCA306.9470014E-526.9879934
TATCATC405.7681245E-726.9879912
ATCATCG351.9794979E-423.1325663
ACGCTGA300.002196100522.5286351
CACTGCT300.002196100522.52863527
CGCTGAG300.002218214622.4899942
GCTGAGT300.002218214622.4899943
GAGTACG404.8259407E-420.2757721
ATCGCAG404.882425E-420.2409936
TCATCGC404.882425E-420.2409934
ACCACTG350.005367344219.3102625
TACCACT350.005367344219.3102624
CTGAGTG350.00542109919.277144
TGAGTGA350.00542109919.277145
Produced by FastQC (version 0.11.2)