FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_21.3520000006090d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_21.3520000006090d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48197
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA1080.2240803369504326No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1050.21785588314625393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.1722098885822769No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC810.16806025271282446No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA810.16806025271282446No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA780.16183579890864577No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA770.15976098097391955No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA710.14731207336556218No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT680.1410876195613835No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA680.1410876195613835No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC610.1265638940182999No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA600.12448907608357367No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA590.12241425814884745No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA550.11411498640994253No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA520.10789053260576384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC306.982547E-527.0207921
ATCTACG307.032258E-526.9927312
TAGCCAG200.00854133726.9927316
TCTACGC307.032258E-526.9927313
TACGCAG307.032258E-526.9927315
CTACGCA307.032258E-526.9927314
AGTCCAG200.00854133726.9927316
TCGCCCA401.8100642E-523.618645
CCAACCC300.00223698322.4939424
TACGCCA300.00223698322.4939425
GATCCAG300.00223698322.4939426
TTGCCAG300.00223698322.4939426
TCTGCGT20000.021.2113227
CAACCAG559.378979E-619.6310776
CCGCCCC350.005466322419.2805214
CAGCCAG501.0108961E-418.8949136
GACCCAG651.8766914E-618.6872756
AGCCAGT1201.8189894E-1217.9951537
TCGCCAG552.0976002E-417.1771936
AACCAGT1154.638423E-1016.4303577
Produced by FastQC (version 0.11.2)