FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_19.352000000608c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_19.352000000608c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33190
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC3501.0545344983428744No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.8586923772220547No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA2190.6598373003916843No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA1930.5815004519433564No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA1920.5784874962338054No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA1670.5031636034950286No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA1590.4790599578186201No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA1540.4639951792708647No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA1480.4459174450135583No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1380.4157878879180476No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1310.39469719795119007No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA1170.35251581801747517No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA1170.35251581801747517No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC1170.35251581801747517No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA1140.3434769508888219No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1100.3314251280506177No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA1090.32841217234106657No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1080.3253992166315155No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1050.3163603495028623No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1040.3133473937933113No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1040.3133473937933113No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA1010.304308526664658No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1010.304308526664658No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC980.2952696595360048No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA870.26212714673094306No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA860.259114191021392No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA850.2561012353118409No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA850.2561012353118409No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA790.23802350105453451No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA780.23501054534498345No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA680.20488098824947273No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA660.19885507683037057No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA630.1898162097017174No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA610.18379029828261526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.1807773425730642No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT580.17475143115396205No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA570.17173847544441095No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG560.16872551973485989No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA550.16571256402530884No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA540.16269960831575775No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA530.15968665260620668No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA530.15968665260620668No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA520.15667369689665564No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA510.15366074118710454No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA490.1476348297680024No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT470.14160891834890027No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA470.14160891834890027No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA470.14160891834890027No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA460.1385959626393492No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA440.13257005122024706No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA430.129557095510696No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT430.129557095510696No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA420.12654413980114493No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.12353118409159385No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA390.11750527267249172No Hit
GAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA380.11449231696294064No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA380.11449231696294064No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.10846640554383852No Hit
TATCTACGCAGAGTGAATGGGCTCTGAAAAAAA360.10846640554383852No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA350.10545344983428744No Hit
CTGAACGTACTCTGCAAAAAAAAAAAAAAAAAA340.10244049412473637No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA340.10244049412473637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT504.7330104E-927.0696961
TAAATGG200.00851079626.9879154
TATCATC504.8894435E-926.9879152
ATCATCG504.8894435E-926.9879153
TCATCGC504.8894435E-926.9879154
TACGCAG551.3491444E-824.534475
TCGCAGA551.3491444E-824.534477
CTACGCA551.3491444E-824.534474
ATCGCAG551.3491444E-824.534476
CATCGCA551.3491444E-824.534475
ACGCTGA300.002185684222.558081
TATCTAC603.290188E-822.558081
GAGTAAA300.002185684222.558081
CGCTGAG300.002224603722.489932
AAACAAT300.002224603722.489936
ATCTACG603.398236E-822.489932
TCTACGC603.398236E-822.489933
AGTAAAT300.002224603722.489932
CTGAATG350.005436508519.2770846
GCGTTGA3800.019.20463627
Produced by FastQC (version 0.11.2)