FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_18.352000000608af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_18.352000000608af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18846
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA2571.363684601506951No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA2071.0983763132760267No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1360.7216385439881141No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA1110.588984399872652No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC970.5146980791679933No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA890.47224875305104536No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA880.46694258728642685No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC690.36612543775867556No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA660.35020694046482015No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA640.33959460893558313No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA620.32898227740634617No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA560.2971452828186353No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA550.2918391170540168No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA540.28653295128939826No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA540.28653295128939826No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC480.2546959567016874No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA470.24938979093706887No Hit
GTGAATGGGGGTATCCAAAAAAAAAAAAAAAAA460.24408362517245036No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA450.2387774594078319No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA450.2387774594078319No Hit
CCAATGCACAAAAAAAAAAAAAAAAAAAAAAAA420.22285896211397643No Hit
ACGCAGAGTGAATGGGTATCCAAAAAAAAAAAA400.21224663058473947No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC380.2016342990555025No Hit
GTGAATGGGTATCCAATAAAAAAAAAAAAAAAA380.2016342990555025No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC370.196328133290884No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC350.18571580176164704No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC350.18571580176164704No Hit
AACGCAGAGTGAATGGGGTATCCAAAAAAAAAA320.16979730446779157No Hit
ATCCAATGCACGTACTCAAAAAAAAAAAAAAAA310.16449113870317308No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC300.1591849729385546No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC300.1591849729385546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.15387880717393612No Hit
GTGAATGGGTATCCAATGCACGAAAAAAAAAAA290.15387880717393612No Hit
GAGGTATCCAATGCACGTACTCTGCGTTGATAC280.14857264140931764No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC280.14857264140931764No Hit
GTGAATGGGGTATCCAATGCACGTACAAAAAAA280.14857264140931764No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA280.14857264140931764No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA280.14857264140931764No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC270.14326647564469913No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC260.13796030988008065No Hit
GTGAATGGGGTATCCAATGCACGTAAAAAAAAA250.13265414411546217No Hit
GTGAATGGGGTATCCAATGCACAAAAAAAAAAA250.13265414411546217No Hit
CCAATGCACGTACAAAAAAAAAAAAAAAAAAAA250.13265414411546217No Hit
GAGTGAATGGGTATCCAAAAAAAAAAAAAAAAA240.1273479783508437No Hit
TATCCAATGCACGTACTCTGCGTTGATACCACT240.1273479783508437No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAAAAA240.1273479783508437No Hit
ACGCAGAGTGAATGGGTATCCAATGCACGTACT230.12204181258622518No Hit
ACGCAGAGTGAATGGGTATCCAATGCACAAAAA210.11142948105698822No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC210.11142948105698822No Hit
ATCCAATGCACGTAAAAAAAAAAAAAAAAAAAA210.11142948105698822No Hit
ATCCAATGCACAAAAAAAAAAAAAAAAAAAAAA210.11142948105698822No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA200.10612331529236974No Hit
ACGCAGAGTGAATGGGGTATCCAATGCACGTAC190.10081714952775125No Hit
AACGCAGAGTGAATGGGTATCCAAAAAAAAAAA190.10081714952775125No Hit
GTGAATGGGGTATCCAATGCACGTACTCTGCGT190.10081714952775125No Hit
AACGCAGAGTGAATGGGTATCCAATGCACGTAC190.10081714952775125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA350.005337075319.2857156
TCTGCGT5900.018.76271227
GTTGATA851.2475302E-615.88235426
TATCAAC851.2475302E-615.8823541
TGGGGTA1600.015.18756
CGTTGAT902.2637832E-615.00000125
CTCTGCG7550.014.8410626
ATGGGGT1557.2759576E-1214.8064515
TTGATAC953.9705756E-614.21052627
ACTCTGC8000.014.17525
CCGGTAT1151.2371856E-714.0869567
GGGGTAT2050.013.82926757
GTACTCT8300.013.523
GCGTTGA1006.7549518E-613.524
AGGTATC1601.9645086E-1013.58
TACTCTG8400.013.524
ACGGTAT600.0071943913.57
ATCAACG1051.11798545E-512.8571422
CGTACTC9100.012.60989122
ACGTACT9200.012.47282621
Produced by FastQC (version 0.11.2)