FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_14.35200000060835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_14.35200000060835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22509
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA7173.1853925096628015No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC2761.2261761961881914No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA1800.7996801279488205No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA1400.6219734328490826No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.5553334221866809No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.4709227420143054No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA1060.4709227420143054No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA990.4398240703718513No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA950.4220534008618775No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA940.41761073348438404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.4042827313519037No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA730.32431471855702165No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA670.297658714292061No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC630.2798880447820872No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA630.2798880447820872No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT610.27100271002710025No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA600.2665600426496068No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC570.2532320405171265No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA530.2354613710071527No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA510.2265760362521658No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA500.22213336887467236No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA500.22213336887467236No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA490.2176907014971789No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA490.2176907014971789No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.21324803411968546No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA470.20880536674219202No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA450.19992003198720512No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA440.19547736460971166No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.19103469723221822No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA420.18659202985472478No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT420.18659202985472478No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCATA390.17326402772224445No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA390.17326402772224445No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA370.16437869296725754No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA370.16437869296725754No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA370.16437869296725754No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA360.1599360255897641No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT360.1599360255897641No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA350.15549335821227064No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA340.1510506908347772No Hit
CAACTCAGAGTGAATGGGCAACAGAGCAAAAAA330.14660802345728374No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCAAA320.1421653560797903No Hit
CCCCCGCAACAGAGCATACGTACTCTGCGTTGA320.1421653560797903No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC320.1421653560797903No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.13772268870229687No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA310.13772268870229687No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA300.1332800213248034No Hit
CAACAGAGCATACGTACAAAAAAAAAAAAAAAA290.12883735394730997No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA290.12883735394730997No Hit
ACAGAGCATACGTACTCAAAAAAAAAAAAAAAA290.12883735394730997No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA280.12439468656981652No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC280.12439468656981652No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA280.12439468656981652No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT280.12439468656981652No Hit
GTGCAACAGAGCATACGTACTCTGCGTTGATAC280.12439468656981652No Hit
GAGTGAATGGGCAACAGAGCATACGTACTCAAA270.11995201919232307No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA270.11995201919232307No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA260.11550935181482963No Hit
GGGCAACAGAGAAAAAAAAAAAAAAAAAAAAAA250.11106668443733618No Hit
GCATACGTAAAAAAAAAAAAAAAAAAAAAAAAA240.10662401705984273No Hit
GAGCATACAAAAAAAAAAAAAAAAAAAAAAAAA240.10662401705984273No Hit
CCTCGCAACAGAGCATACGTACTCTGCGTTGAT240.10662401705984273No Hit
GCATACGTACAAAAAAAAAAAAAAAAAAAAAAA240.10662401705984273No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA240.10662401705984273No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA230.10218134968234928No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA230.10218134968234928No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA230.10218134968234928No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA230.10218134968234928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00845531726.9866371
AACTCAG200.00845531726.9866372
GTATCAT300.002203029822.4888651
TATCATC300.002203029822.4888652
TTGATAC707.0427624E-515.45535827
TATCAAC2550.014.2870441
GGTATCA852.2965674E-514.2870441
ATCAACG2650.013.747912
GTTGATA802.1951395E-413.523437526
CGTTGAT1602.0190782E-1013.523437527
TCAACGC2700.012.9935673
CAACGCA2850.012.3096944
GTATCAA1605.702241E-710.9633221
GCGTTGA2006.850314E-910.8187526
AACGCAG3250.010.3794775
ACTCAAA1301.0194551E-410.37947711
TACTCAA1301.0194551E-410.37947710
TCTGCGT4650.010.17893127
TGCGTTG2203.027344E-89.83522725
GTACTCA1402.1565105E-49.6380849
Produced by FastQC (version 0.11.2)