FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_13.3520000006081b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_13.3520000006081b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17647
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA1811.0256700855669518No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC1370.776335921119737No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC1300.7366691222304075No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1000.5666685555618518No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA940.5326684422281408No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA890.5043350144500481No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA870.49300164333881114No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA790.447668158893863No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC790.447668158893863No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC520.29466764889216296No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA420.2380007933359778No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA410.23233410778035926No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA370.20966736555788518No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC360.20400068000226668No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA340.19266730889102962No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA300.17000056666855556No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT280.15866719555731854No Hit
GTACGTGACTCTAAAAAAAAAAAAAAAAAAAAA280.15866719555731854No Hit
ACACCATAGAGTCAAAAAAAAAAAAAAAAAAAA260.14733382444608148No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA260.14733382444608148No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA260.14733382444608148No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA260.14733382444608148No Hit
GTGAATGGGGAGTACGTGACTCTAAAAAAAAAA240.13600045333484445No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA240.13600045333484445No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13033376777922592No Hit
ACACCATAGAGAAAAAAAAAAAAAAAAAAAAAA230.13033376777922592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12466708222360742No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA220.12466708222360742No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA220.12466708222360742No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTA220.12466708222360742No Hit
GAGTGAATGGGCACCATAGAAAAAAAAAAAAAA210.1190003966679889No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA210.1190003966679889No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA200.11333371111237037No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA200.11333371111237037No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA200.11333371111237037No Hit
CCCCACCATAGAGTCACGTACTCTGCGTTGATA190.10766702555675187No Hit
GTCACGTACTCAAAAAAAAAAAAAAAAAAAAAA190.10766702555675187No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA190.10766702555675187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG300.002193934322.471592
TATCTAC300.002193934322.471591
ATGGTGA350.00536272419.2613646
CATCACC350.00536272419.2613646
TGATACC450.001021137318.0827
TTGATAC450.001021137318.0826
TATCAAC951.1816155E-817.0311011
ACTCTAT901.2218334E-716.4791661
ATCAACG1002.2269887E-816.1795462
TCTATGG952.261786E-715.6118433
CTCTATG1004.0498526E-714.8312512
CTATGGT1004.0498526E-714.8312514
TCAACGC1107.1953764E-814.7086773
TATGGTG953.9872266E-614.1925855
ATCACCA700.001262348313.4829557
CAACGCA1202.0871448E-713.4829544
AACGCAG1305.529855E-712.4458055
GTTGATA905.691966E-412.05333327
TCTGCGT4950.012.05333327
CGTTGAT958.9663244E-411.41894726
Produced by FastQC (version 0.11.2)