FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_12.352000000607f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_12.352000000607f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29951
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3521.1752529130913827No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2770.92484391172248No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA2700.9014724049280491No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA2590.8647457513939435No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA2420.8079863777503256No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA2250.7512270041067076No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA1680.5609161630663417No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA1410.4707689225735368No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA1350.45073620246402457No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC1340.44739741577910586No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA1250.4173483356148376No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA1240.41400954892991887No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA1180.39397682882040663No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA980.32720109512203266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.3071683750125205No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA920.3071683750125205No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.26710293479349606No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA770.2570865747387399No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.24707021468398385No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA740.24707021468398385No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA680.22703749457447164No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA620.20700477446495943No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA620.20700477446495943No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA620.20700477446495943No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA590.19698841441020332No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA530.17695569430069114No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC520.17361690761577242No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA520.17361690761577242No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT510.17027812093085373No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA510.17027812093085373No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA500.16693933424593502No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA490.16360054756101633No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA490.16360054756101633No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA470.15692297419117893No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA450.15024540082134152No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA440.14690661413642284No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA430.14356782745150412No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA430.14356782745150412No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGAAAA410.13689025408166672No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA400.13355146739674803No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA400.13355146739674803No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA390.13021268071182932No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGATAAA390.13021268071182932No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA380.12687389402691063No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC370.12353510734199193No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA350.11685753397215452No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA350.11685753397215452No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA340.11351874728723582No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG330.11017996060231712No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA330.11017996060231712No Hit
GTGAATGGGATTCACGTACTCTGCGTAAAAAAA320.10684117391739842No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA320.10684117391739842No Hit
GGATTCACGTACAAAAAAAAAAAAAAAAAAAAA310.10350238723247972No Hit
ACGCAGAGTGAATGGGGCGTAAGGAAAAAAAAA310.10350238723247972No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA310.10350238723247972No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGAAAAA300.10016360054756102No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA300.10016360054756102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG200.00841963827.0386561
GTATCAT405.6810495E-727.0386561
CAACTCA306.8635636E-527.0386541
GTATGGG200.00848911626.993293
TGTATGG200.00848911626.993292
TATCATC451.6106023E-623.9940342
ATCATCG451.6106023E-623.9940343
CATCGCA451.6106023E-623.9940345
AACTCAG351.9783183E-423.1371042
TCATCGC504.023301E-621.5946314
ACTCAGA404.8795025E-420.2449683
TCAGAGT404.8795025E-420.2449685
CTCAGAG404.8795025E-420.2449684
ATGCGTA559.181169E-619.6314837
TCGCAGA559.181169E-619.6314837
TATCTAC350.005365199419.3133241
TACGCAG350.00541774919.280925
CTACGCA350.00541774919.280924
TATCAAC2150.018.2353731
ATCGCAG601.9443774E-517.9955256
Produced by FastQC (version 0.11.2)