FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_10.352000000607a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_10.352000000607a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29292
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA4101.3996995766762257No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3001.0241704219582137No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA2450.8364058445992079No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1730.5906049433292366No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1640.5598798306704902No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1230.41990987300286764No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1020.3482179434657927No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1000.34139014065273793No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA740.25262870408302607No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA720.24580090126997134No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA630.21507578861122492No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA630.21507578861122492No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA590.20142018298511538No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC570.19459238017206062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.17752287313942372No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA450.1536255632937321No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT440.1502116618872047No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA430.1467977604806773No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT410.13996995766762255No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA410.13996995766762255No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA410.13996995766762255No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA350.11948654922845829No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT350.11948654922845829No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA350.11948654922845829No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC340.1160726478219309No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA340.1160726478219309No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA330.11265874641540352No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT330.11265874641540352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC300.002147650822.61494326
TACCACT300.002147650822.61494327
TATCAAC1550.017.3884221
ATCAACG1650.016.334582
GATACCA756.7891688E-616.28275927
TCAACGC1750.015.4011743
GCGTTGA2750.015.29592527
TGATACC801.2527276E-515.26508526
CAACGCA1950.014.5126454
GTATCAA700.001303158613.4760271
TTGATAC956.3309846E-512.8548125
AACGCAG2400.011.7915245
GTTGATA1153.7523263E-410.6191924
TGCGTTG4200.010.01518826
ACGCAGA3400.09.5124896
CTGCGTT4500.09.3475125
GCAGAGT3650.09.2301568
CAGAGTG3850.09.1006949
CGTTGAT1350.00163607729.04597723
CGCAGAG3750.08.9840177
Produced by FastQC (version 0.11.2)