FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate8_1.35200000060694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate8_1.35200000060694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54561
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA13802.5292791554406993No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA10581.9391140191712029No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA5681.0410366378915343No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA5240.9603929546745844No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA4960.9090742471728891No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA4540.832096185920346No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA3480.637818221806785No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.6341525998423783No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA3280.601162002162717No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA3150.5773354593940727No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA3050.5590073495720387No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA3030.5553417276076319No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA2790.5113542640347502No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA2510.4600355565330548No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.45636993456864794No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA2450.4490386906398343No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA2450.4490386906398343No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA2170.397719983138139No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.39405436117373216No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA2040.3738934403694947No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT2040.3738934403694947No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA2010.3683950074228845No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA1930.35373251956525725No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.3518997085830538No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA1920.3518997085830538No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT1890.34640127563644363No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA1690.3097450559923755No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA1550.28408570224152785No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1540.28225289125932446No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA1520.2785872692949176No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT1490.2730888363483074No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA1480.271256025366104No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA1320.2419310496508495No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA1310.2400982386686461No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.23276699473983248No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1230.22543575081101888No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC1190.21810450688220523No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA1180.2162716959000018No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA1180.2162716959000018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.2052748300067814No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA1080.19794358607796778No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA1080.19794358607796778No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1050.19244515313135757No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.19244515313135757No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA1020.18694672018474734No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1010.18511390920254395No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA990.1814482872381371No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA990.1814482872381371No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA950.1741170433093235No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC920.1686186103627133No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA880.16128736643389968No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA840.15395612250508606No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA830.15212331152288264No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA830.15212331152288264No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA800.14662487857627243No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA780.14295925661186562No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA750.1374608236652554No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA750.1374608236652554No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA730.13379520170084858No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA730.13379520170084858No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1301295797364418No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA590.1081358479500009No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG590.1081358479500009No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA580.1063030369677975No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA580.1063030369677975No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC560.1026374150033907No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA560.1026374150033907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT200.00855113426.9926534
ATTAGCA200.00855113426.9926537
TATCATC1200.026.9926532
TAGAGTG200.00855113426.9926535
GTATCAT1250.025.9367661
TCTACGC1350.024.9931983
TACGCAG1350.024.9931985
CTACGCA1350.024.9931984
TATCTAC1300.024.9391981
ACGCTGA601.2660166E-924.7660061
CGCTGAG551.3882527E-824.5387762
ATCTACG1400.024.1005822
CTGAGTG451.6525973E-623.9934714
GCTGAGT451.6525973E-623.9934713
TCGCAGA1350.023.993477
ATCATCG1350.023.993473
ATCGCAG1350.023.993476
TCATCGC1350.023.993474
CATCGCA1400.023.136565
AACATGA300.002240768922.4938776
Produced by FastQC (version 0.11.2)