FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_96.3520000006066d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_96.3520000006066d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16926
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA3291.943755169561621No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA940.5553586198747489No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA820.4844617747843554No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA690.40765685926976253No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA650.38402457757296465No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA530.3131277324825712No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA530.3131277324825712No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA500.2954035212099728No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA430.2540470282405766No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA420.24813895781637718No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC350.20678246484698098No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA330.19496632399858208No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA330.19496632399858208No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA320.1890582535743826No Hit
TATCAACGCAGAGTGAATGGGGTTCCACAAAAA320.1890582535743826No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA290.17133404230178423No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCAAA270.15951790145338532No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA250.1477017606049864No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA250.1477017606049864No Hit
CGTTAAGCGTGAAAAAAAAAAAAAAAAAAAAAA250.1477017606049864No Hit
AACGCAGAGTGAATGGGTTCCACAAAAAAAAAA230.1358856197565875No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA220.12997754933238803No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA220.12997754933238803No Hit
CCCCCGTTCCACGCTTAACGTACTCTGCGTTGA220.12997754933238803No Hit
GTGAATGGGGGTTCCACAAAAAAAAAAAAAAAA200.11816140848398912No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA200.11816140848398912No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA200.11816140848398912No Hit
ACGCAGAGTGAATGGGTTCCACGAAAAAAAAAA190.11225333805978967No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA190.11225333805978967No Hit
GTTCCACGCTTAACGTACTCTTCGTTGATACCA180.10634526763559021No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT180.10634526763559021No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA180.10634526763559021No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA170.10043719721139076No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA170.10043719721139076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA601.8589104E-518.00000227
TTGATAC601.8589104E-518.00000225
GTTGATA653.698886E-516.61538524
TGATACC653.698886E-516.61538526
CGTTGAT653.698886E-516.61538523
GTATCAA1153.8380676E-1016.4347841
TATCAAC1303.560308E-813.51
ATCAACG1451.018816E-813.0344832
GCGTTGA1305.395286E-712.46153827
TCAACGC1552.5929694E-812.1935483
AATGGGT1001.02178834E-412.154
ATGGGGG800.00340197111.81255
TGCGTTG2201.4551915E-1111.65909127
CAACGCA1656.198752E-811.4545464
AATGGGA959.271675E-411.3684214
CTGCGTT2407.6397555E-1110.68750126
AACGCAG1904.3591717E-79.9473685
ACGCAGA3003.6379788E-129.9000011
TGAATGG5250.09.7714292
CAGAGTG2654.6929927E-109.6792454
Produced by FastQC (version 0.11.2)