FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_94.35200000060637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_94.35200000060637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95254
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA4600.48291935246813783No Hit
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC3640.382136183257396No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA3070.32229617653851805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.1700715980431268No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.1616730006088983No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1260.13227790958909863No Hit
GTGAATGGGTACGTTACTGCAAAAAAAAAAAAA1250.13122808490982008No Hit
GTGAATGGGAGTACGTTACTGCAAAAAAAAAAA1180.12387931215487014No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA1170.12282948747559157No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA1160.12177966279631303No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1130.11863018875847733No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.10288281856929893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG200.0085144427.0368827
GTATCAT554.765752E-1026.9942151
GTATGGG200.008580347526.9942133
GTATCTA200.008580347526.9942131
CAACACT200.008580347526.9942134
TGGGCCA200.00860240726.980026
AAACCCG300.00225227922.4951781
CAACTCA300.00225227922.4951781
TATCATC603.5643097E-822.4951782
ATCATCG603.5643097E-822.4951783
TCGCAGA603.5843186E-822.483357
ATCGCAG603.5843186E-822.483356
TGCGTTG9550.020.95004327
TCATCGC658.3126E-820.764784
CATCGCA658.359166E-820.7538625
TGTCTCC559.577574E-619.6218346
TAGAGTG559.577574E-619.6218345
CTCACTC350.005519969419.2714445
ATACCAC501.0114265E-418.92581727
GTAGAGT602.018456E-517.9961434
Produced by FastQC (version 0.11.2)