FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_92.352000000605f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_92.352000000605f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62242
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA2110.3389993894797725No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.3213264355258507No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA1990.3197198033482214No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC1720.2763407345522316No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1590.2554545162430513No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA1150.18476270042736417No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.14941679251952059No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA930.14941679251952059No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA920.14781016034189132No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA910.14620352816426208No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA890.14299026380900356No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA860.1381703672761158No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA860.1381703672761158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.12853057421034028No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA780.12531730985508177No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA750.12049741332219402No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA750.12049741332219402No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA710.114070884611677No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA670.10764435590115999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCT200.00855645226.99517813
TACTCAG200.00855645226.9951785
GTTGCTA200.00855645226.99517814
TTGCTAA200.00855645226.99517815
GAGTGTC307.0605514E-526.99517611
GTATCAT604.3655746E-1126.9951761
AGTGTCT307.0605514E-526.99517612
CAACTCA651.1823431E-1024.9186251
GTGTCTA352.0114689E-423.13872313
AGAGTGT352.0114689E-423.13872310
TATCATC653.250534E-922.8420732
TGTCTAA300.002243123722.4959814
TCTAAAC300.002243123722.4959816
GTCTAAA300.002243123722.4959815
ATCATCG707.6452125E-921.2104973
TCATCGC707.6452125E-921.2104974
ACGCTGA454.519638E-520.996251
TATCTAC454.519638E-520.996251
TGAGTGA454.519638E-520.996255
ATCGCAG851.5097612E-1020.643376
Produced by FastQC (version 0.11.2)