FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_88.3520000006056e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_88.3520000006056e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47117
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA4240.8998875140607425No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA2780.5900205870492604No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2550.5412059341638898No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA1910.4053738565698155No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1510.32047880807351914No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1320.2801536600377783No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA1300.2759089076129635No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1300.2759089076129635No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1280.2716641551881486No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1260.2674194027633338No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1220.2589298979137042No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1110.23558375957722266No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA1070.227094254727593No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC1030.21860474987796336No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA950.20162574017870408No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA930.19738098775388926No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA850.18040197805462996No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA820.17403484941740774No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA750.15917821593055584No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA740.15705583971814843No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC680.14432158244370397No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA620.1315873251692595No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA620.1315873251692595No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA560.11885306789481505No Hit
ACGCAGAGTGAATGGGTCAAGCAAAAAAAAAAA550.11673069168240761No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA540.11460831547000022No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC540.11460831547000022No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA530.11248593925759282No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA520.11036356304518538No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA510.10824118683277799No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA480.10187405819555576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA653.1122909E-922.89661627
ATCGTCA404.962575E-420.2300956
TGATACC901.3061072E-716.53644626
TATCAAC2600.016.0803341
TCAACGC2900.014.4168483
ATCAACG2900.014.4168482
TCGTCAA751.3477574E-414.3858467
ACTCAAA853.8928672E-412.69339312
CAACGCA3300.012.6693534
GTACTCA750.00221284912.58761510
TACTCAA750.00221284912.58761511
TCTGCGT6450.012.58587827
AACGCAG4000.010.4522165
CGTCAAG4200.010.2756048
GTTGATA1452.7298067E-510.27494224
TTGATAC1452.7298067E-510.27494225
CTCTGCG7800.010.23409426
ACGTCAA1258.7695464E-49.7104467
ACTCTGC8350.09.57018425
CGTTGAT1608.045695E-59.311666523
Produced by FastQC (version 0.11.2)