FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_87.35200000060545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_87.35200000060545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45974
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2340.5089833384086657No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA1910.41545221211989386No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1300.28276852133814767No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC1120.24361595684517334No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1110.24144081437334144No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1010.21968938965502238No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT990.2153391047113586No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT990.2153391047113586No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA980.21316396223952666No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA970.2109888197676948No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA960.2088136772958629No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA940.20446339235219907No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA930.20228824988036717No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA910.19793796493670335No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA850.18488711010571193No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA850.18488711010571193No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA790.1718362552747205No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA760.16531082785922477Illumina Single End PCR Primer 1 (100% over 29bp)
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA750.1631356853873929No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.15878540044372907No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT670.14573454561273763No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA670.14573454561273763No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA650.14138426066907384No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA640.1392091181972419No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA620.1348588332535781No Hit
GACGAAGTACGTACTCAAAAAAAAAAAAAAAAA610.13268369078174622No Hit
GTACGTACTCTGCGTTGATACCAAAAAAAAAAA600.1305085483099143No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA600.1305085483099143No Hit
GTACGTACTCTGCGAAAAAAAAAAAAAAAAAAA540.11745769347892288No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA540.11745769347892288No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA530.11528255100709096No Hit
GTGAATGGGAGCTGACGAAGTACAAAAAAAAAA520.11310740853525905No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA510.11093226606342715No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.11093226606342715No Hit
CAGCTGACGAAGTACGTACTCTGCGTTGATACC490.10658198111976333No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA480.10440683864793145No Hit
GAGTACGTACTCTGCGAAAAAAAAAAAAAAAAA480.10440683864793145No Hit
GTGAATGGGAGCTGACGAAGTACGTACTCAAAA470.10223169617609953No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA460.10005655370426764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGT200.00852603727.014
ATCTACG356.4033848E-627.02
TCTACGC307.012326E-527.03
TACGCAG307.012326E-527.05
TATCATC200.00852603727.02
CTACGCA307.012326E-527.04
TAAGTAC257.713241E-426.99999816
CAACTCA351.9978662E-423.1428591
TATCTAC351.9978662E-423.1428591
CTCGGTG350.005454000619.2857159
AACTCAG450.001088309518.02
ACTCAGA450.001088309518.03
TCAGAGT450.001088309518.05
CTCAGAG450.001088309518.04
CACAGCT552.090591E-417.1818187
TGCGTTG5650.016.2477927
TATCAAC3250.015.7846161
ATCAACG3500.014.6571442
TCAACGC3450.014.4782613
TCCAGCT700.001305681713.56
Produced by FastQC (version 0.11.2)