FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_84.352000000604e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_84.352000000604e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40934
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC2950.7206723017540431No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1870.4568329506034104No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA1780.4348463380075243No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA1620.3957590267259491No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1010.24673865246494356No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA970.23696682464454977No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.2052083842282699No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA830.20276542727317146No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA820.200322470318073No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA790.19299359945277764No Hit
GTGAATGGGGACTGTCGAAAAAAAAAAAAAAAA780.1905506424976792No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA760.1856647285874823No Hit
CCACTGTCGTTCTGACGTACTCTGCGTTGATAC730.17833585772218694No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.17589290076708847No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA680.16612107294669468No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA670.16367811599159623No Hit
ACGCAGAGTGAATGGGACTGTCGAAAAAAAAAA670.16367811599159623No Hit
GAGTGAATGGGACTGTCGAAAAAAAAAAAAAAA620.15146333121610397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14169150339571016No Hit
GTTCTGACGTACTCAAAAAAAAAAAAAAAAAAA580.14169150339571016No Hit
ACGCAGAGTGAATGGGACTGTCGTAAAAAAAAA560.13680558948551327No Hit
GAGTGAATGGGACTGTCGTAAAAAAAAAAAAAA550.1343626325304148No Hit
GAATGGGACTGTCGAAAAAAAAAAAAAAAAAAA500.12214784775492257No Hit
TATCAACGCAGAGTGAATGGGACTGTCGTAAAA500.12214784775492257No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11726193384472565No Hit
TATCAACGCAGAGTGAATGGGACTGTCGAAAAA460.11237601993452875No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA450.1099330629794303No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1099330629794303No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10749010602433184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA257.669896E-427.0171591
GTATCAT306.963296E-527.0171591
TATCTAC257.669896E-427.0171591
CTGAGTG200.0085397426.9840894
AGTACTG200.0085397426.9840896
GCTGAGT200.0085397426.9840893
TGAGTGA200.0085397426.9840895
CGCTGAG257.7251426E-426.9840872
ATCTACG257.7251426E-426.9840872
TCTACGC257.7251426E-426.9840873
CTACGCA257.7251426E-426.9840874
TATCATC352.0005894E-423.129222
TCGCAGA352.0005894E-423.129227
ATCGCAG352.0005894E-423.129226
TGTCTTT300.002235359522.4867412
GTCTTTC300.002235359522.4867413
CTGTCTT350.005462486319.2743511
AAGCACT350.005462486319.274355
TCTGCGT14350.018.9446427
AGCCACT450.001089815317.9893915
Produced by FastQC (version 0.11.2)