FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_79.35200000060453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_79.35200000060453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62059
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA2880.4640745097407306No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA2530.4076765658486279No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA2400.3867287581172755No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2330.37544916933885497No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1930.31099437631930904No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1830.29488067806442253No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1810.2916579384134453No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA1430.23042588504487665No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1390.22398040574292205No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1360.21914629626645613No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA1240.19980985836059234No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA1150.1853075299311945No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA1120.18047342045472856No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1080.17402794115277398No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA970.15630287307239885No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA970.15630287307239885No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA960.1546915032469102No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA920.1482460239449556No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA890.14341191446848967No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA870.14018917481751236No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA820.13213232569006914No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT790.12729821621360318No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA780.12568684638811453No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA740.11924136708615994No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA730.1176299972606713No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA730.1176299972606713No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA720.11601862743518265No Hit
GAGTACGTCAGACAAAAAAAAAAAAAAAAAAAA720.11601862743518265No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.11601862743518265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.114407257609694No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA710.114407257609694No Hit
GTACGTAGACATCGCAAAAAAAAAAAAAAAAAA680.10957314813322806No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA680.10957314813322806No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA670.10796177830773941No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA670.10796177830773941No Hit
GTACGTCAGACATCAAAAAAAAAAAAAAAAAAA660.10635040848225076No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA650.10473903865676211No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT650.10473903865676211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.008548791527.02
ACGCTGA200.008548791527.01
ATCTACG405.92554E-727.02
GTATCAT356.4512387E-627.01
TCTACGC405.92554E-727.03
TATCTAC405.92554E-727.01
CTACGCA405.92554E-727.04
ATCAACT200.008548791527.04
GACTTGC257.744099E-427.06
TGAGTGA200.008548791527.05
TATCATC451.654882E-624.02
TACGCAG504.1329113E-621.65
ATCATCG559.429024E-619.6363643
GTAGAGT350.00547525519.2857154
TAGAGTG350.00547525519.2857155
TCATCGC601.9963863E-518.0000024
TCGCAGA653.96999E-516.6153857
ATCGCAG653.96999E-516.6153856
GTATCAA2750.014.7272741
TCTGCGT8300.014.31325327
Produced by FastQC (version 0.11.2)