FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_78.35200000060439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_78.35200000060439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40454
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA3770.9319226776091363No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3460.8552924309091807No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1910.4721411974094033No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1620.4004548375933159No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1490.36831957284817324No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA1480.3658476294062392No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1470.36337568596430514No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1420.3510159687546349No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1390.34360013842883275No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1290.31888070400949226No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1270.31393681712562416No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA1020.25213823107727296No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA980.24225045730953673No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA930.22989074009986651No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA900.22247490977406437No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA760.18786770158698768No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA750.18539575814505363No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA730.18045187126118553No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA690.17056409749344933No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA680.16809215405151529No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA660.16314826716764722No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA650.16067632372571314No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA640.1582043802837791No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA630.15573243684184507No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA630.15573243684184507No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT560.13842883274830672No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA540.13348494586443863No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA540.13348494586443863No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA540.13348494586443863No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC530.13101300242250458No Hit
GAGTGAATGGGGTTCGTCAAAAAAAAAAAAAAA530.13101300242250458No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA510.12606911553863648No Hit
GTACGTAGTGTGACGAAAAAAAAAAAAAAAAAA510.12606911553863648No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.12112522865476837No Hit
GAGTGAATGGGTTCGTCAAAAAAAAAAAAAAAA490.12112522865476837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.11370939832896625No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA460.11370939832896625No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT450.11123745488703218No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACAAAA450.11123745488703218No Hit
GAGTGAATGGGTTCGTCACAAAAAAAAAAAAAA410.10134968111929599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGT350.005468913419.2698026
TATCAAC2600.017.639281
GATACCA952.3726352E-715.67688827
TGATACC1054.3188265E-815.47329226
TCTGCGT7400.014.63689827
ATCAACG3150.014.5594052
TCAACGC3200.014.3319153
GTTGATA1151.3175122E-714.12778824
TTGATAC1202.2152926E-713.53913125
CGTTGAT1202.2152926E-713.53913123
CAACGCA3400.013.4888624
ATGTTCG1102.6756315E-411.0363417
AATGGGC1000.001512826110.79108916
CTCTGCG10100.010.72406426
AACGCAG4100.010.5278915
TGGGTTC2051.17652235E-810.5278916
TGTTCGT2707.2759576E-1210.4913378
ACGTTCG1050.002271206810.2772277
ACTCTGC11100.09.75793225
ATGGGTT1957.336075E-79.6843115
Produced by FastQC (version 0.11.2)