FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_76.352000000603f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_76.352000000603f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38592
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA5041.3059701492537312No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT2580.6685323383084577No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2520.6529850746268656No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA1660.4301409618573798No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1340.3472222222222222No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1230.3187189054726368No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1210.31353648424543945No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1200.3109452736318408No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1180.30576285240464346No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1170.3031716417910448No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1030.26689469320066334No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA940.2435737976782753No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC940.2435737976782753No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA930.2409825870646766No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA890.23061774461028192No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA870.22543532338308458No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA770.19952321724709784No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA740.19174958540630183No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA730.18915837479270314No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA680.1762023217247098No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA630.1632462686567164No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT620.16065505804311775No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA620.16065505804311775No Hit
ACGCAGAGTGAATGGGATCTCGTCAAAAAAAAA580.15029021558872308No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA570.14769900497512436No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA570.14769900497512436No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA540.13992537313432835No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1373341625207297No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA530.1373341625207297No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC520.134742951907131No Hit
GATCTCGTCCTCTACGTACTCTGCGTTGATACC510.13215174129353235No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT480.12437810945273632No Hit
GAGTACGTAGAGGACGAAAAAAAAAAAAAAAAA460.11919568822553897No Hit
GGTACGTAGAGGACGAAAAAAAAAAAAAAAAAA460.11919568822553897No Hit
GTGAATGGGATCTCGTCCTAAAAAAAAAAAAAA450.11660447761194029No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA450.11660447761194029No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA450.11660447761194029No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA430.11142205638474294No Hit
ATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAAA430.11142205638474294No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA420.10883084577114428No Hit
GTGAATGGGGATCTCGTCAAAAAAAAAAAAAAA410.1062396351575456No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10364842454394693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.10105721393034825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.00848729727.0142842
ACGCTGA200.00848729727.0142841
TACCACT257.6667237E-427.01428427
CTGAGTG200.008541333526.9792464
GCTGAGT200.008541333526.9792463
TGAGTGA200.008541333526.9792465
GATACCA1251.4606485E-915.12827
TGGGGTA550.004164285514.7159546
AATGGGT657.460156E-414.5272874
GCGTTGA3950.013.6781227
ATGGGGT904.0945008E-513.4896245
TGATACC1451.1814336E-813.0413826
AAATCTC1152.1344476E-612.9031187
TATCAAC3000.012.1564291
GTTGATA1604.6717105E-811.81874924
TTGATAC1604.6717105E-811.81874925
TGCGTTG6550.010.723227527
ATCAACG3400.010.3289922
TCAACGC3600.010.1172183
CTATCTC1258.675738E-49.7125297
Produced by FastQC (version 0.11.2)