FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_72.3520000006037d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_72.3520000006037d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41174
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC4561.107495021129839No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC4521.097780152523437No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2310.5610336620197212No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA2230.541603924806917No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA2020.490600864623306No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA1830.44445523874289594No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1820.44202652159129546No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1620.393452178559285No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1500.3643075727400787No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1450.35216398698207607No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1230.29873220964686453No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1230.29873220964686453No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA1200.29144605819206293No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1190.2890173410404624No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC1160.28173118958566085No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1100.2671588866760577No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1090.2647301695244572No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1060.2574440180696556No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1040.2525865837664546No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA990.24044299800845192No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT980.23801428085685142No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA930.22587069509884877No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA920.22344197794724827No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA900.2185845436440472No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA890.2161558264924467No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA860.20886967503764511No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA810.1967260892796425No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC780.18943993782484092No Hit
ACACTACACAAAAAAAAAAAAAAAAAAAAAAAA740.1797250692184388No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA720.17486763491523777No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA710.17243891776363723No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC700.17001020061203673No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC640.15543789770243358No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC630.15300918055083304No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT590.14329431194443096No Hit
GTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14086559479283042No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC570.1384368776412299No Hit
GTACGTGTGTAGTGTGGAAAAAAAAAAAAAAAA570.1384368776412299No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC570.1384368776412299No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC560.13600816048962938No Hit
TGTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAA530.1287220090348278No Hit
GAGTACGTGTGTAGTGTGAAAAAAAAAAAAAAA500.12143585758002623No Hit
GAGTACGTGTGTAGTGTGGAAAAAAAAAAAAAA500.12143585758002623No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11414970612522465No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA450.1092922718220236No Hit
ACACTACACACAAAAAAAAAAAAAAAAAAAAAA450.1092922718220236No Hit
GTAGTGTGGAAAAAAAAAAAAAAAAAAAAAAAA440.10686355467042308No Hit
GTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10443483751882256No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT420.10200612036722204No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.10200612036722204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCT200.008560942526.970843
GATACCA551.3089448E-824.63869726
ATACCAC603.297282E-822.58546827
TTGATAC2650.020.45476527
TGATACC701.6813829E-719.35897625
GTTGATA3050.017.77217326
TATCAAC2150.016.307951
ATGGGTG1002.4923793E-816.1825035
AACTCCA500.002214396816.1825036
CCTCTCC500.002214396816.1825035
TGGGTGT1002.4923793E-816.1825036
ATGGGAG550.00417932214.7113685
ATCAACG2450.014.3110572
CGCTCCA904.1198364E-513.4854196
TCAACGC2500.013.4854193
TCTGCGT14000.013.26089727
CGTTGAT4150.013.06147725
TGGGGTG1501.9570507E-812.58639056
CAACGCA2900.011.6253614
CTCTGCG16100.011.61538526
Produced by FastQC (version 0.11.2)