FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_71.35200000060354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_71.35200000060354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62841
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA14592.3217326267882434No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA7711.2269060008593116No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA6431.0232173262678823No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA4590.730414856542703No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG4500.7160929966104931No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC3930.6253878837064973No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA3710.5903788927610955No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA2490.39623812479113957No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA2400.3819162648589297No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC2240.356455180535001No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA2110.335768049521809No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC2080.3309940962110724No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA1970.31348960073837145No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.30871564742763485No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA1930.3071243296573893No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA1880.29916774080616154No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1770.28166324533346065No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1750.27848060979296957No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT1740.276889292022724No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC1720.2737066564822329No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA1610.256202161009532No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT1500.23869766553683108No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA1470.2339237122260944No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA1460.23233239445584886No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT1440.2291497589153578No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA1240.197323403510447No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC1190.1893668146592193No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG1170.18618417911872823No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC1160.18459286134848268No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT1090.17345363695676388No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA1050.16708836587578174No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA960.15276650594357188No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA910.14480991709234417No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA900.14321859932209863No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1304880571601343No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA760.12094015053866107No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA760.12094015053866107No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA760.12094015053866107No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.11934883276841554No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA730.11616619722792443No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA720.1145748794576789No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA720.1145748794576789No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA700.11139224391718781No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA690.10980092614694227No Hit
GAGTGAATGGGATAGCAGATACGTACTAAAAAA690.10980092614694227No Hit
GTACGTATCTGCTAAAAAAAAAAAAAAAAAAAA690.10980092614694227No Hit
GTGAATGGGCGGATAGCAGAAAAAAAAAAAAAA650.10343565506596011No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA640.10184433729571458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT554.620233E-1027.0263581
TATCTAC601.2696546E-924.774161
ATCTACG601.2933015E-924.7346482
TCTACGC601.2933015E-924.7346483
TATCATC601.2933015E-924.7346482
ATCATCG653.268724E-922.8319843
CTACGCA653.268724E-922.8319844
TCATCGC653.268724E-922.8319844
TACGCAG707.687049E-921.2011285
CATCGCA707.687049E-921.2011285
CAACTCA1300.020.7895071
AACTCAG1300.020.756352
TCAGAGT1250.020.5072735
TACCACT404.895853E-420.2859727
CTCAGAG1350.019.9875974
ATCGCAG751.700937E-819.787726
ACTCAGA1400.019.2737523
TCGCAGA701.7992897E-719.2737527
TATCAAC5000.017.0266061
ATCAACG5200.015.5672622
Produced by FastQC (version 0.11.2)