FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_69.35200000060310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_69.35200000060310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59601
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2290.38422174124595226No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA1740.2919414103790205No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA1500.2516736296370866No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1330.2231506182782168No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA1220.20469455210483045No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA1110.18623848593144413No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1070.1795271891411218No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA1050.17617154074596064No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1040.17449371654838008No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1000.16778241975805774No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA1000.16778241975805774No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1000.16778241975805774No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA970.16274894716531602No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA930.1560376503749937No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA920.15435982617741312No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC900.15100417778225197No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA880.1476485293870908No Hit
ACGCAGAGTGAATGGGTACGTACTCTGCGTTGA860.14429288099192966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.13758158420160735No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA760.1275146390161239No Hit
GTGAATGGGCCAGTTCAAGGTAAAAAAAAAAAA680.11409204543547927No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.11241422123789868No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA660.11073639704031811No Hit
GTATCAACGCAGAGTGAATGGGTACGTACTCTG640.10738074864515695No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA620.1040251002499958No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT610.10234727605241523No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC610.10234727605241523No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA600.10066945185483464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT257.715013E-427.01513922
ACTTACT257.715013E-427.01513920
CTTACTC257.715013E-427.01513921
GTACTTA257.7530777E-426.99243718
TCACCCA257.7530777E-426.9924375
TGTACCA257.7530777E-426.9924375
GGTACTT257.7530777E-426.99243717
TACTTAC257.7530777E-426.99243719
GTATCAT501.5044316E-724.3136251
ATACCAG300.002243306522.4936986
TATCATC553.8051985E-722.0847232
CTTGCCA404.9602415E-420.244335
TCGCAGA608.78621E-720.2443287
ATCATCG608.78621E-720.2443283
ATCGCAG608.78621E-720.2443286
TCATCGC608.78621E-720.2443284
CATCGCA608.78621E-720.2443285
TATCCAG350.005481574719.2803136
AGGTACT350.005481574719.28031316
TTGCCCA350.005481574719.2803135
Produced by FastQC (version 0.11.2)