FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_63.35200000060261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_63.35200000060261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54078
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA4540.8395280890565479No Hit
CAGCTATGCCAACGTACTCTGCGTTGATACCAC4380.8099411960501499No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2290.4234624061540737No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2220.41051814046377455No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2140.39572469396057547No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1990.36798698176707717No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1870.3457968120122785No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1720.3180590998187803No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1710.3162099190058804No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA1510.2792263027478827No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1430.2644328562446836No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1330.24594104811568474No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC1090.2015607086060875No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA1040.19231480454158809No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT980.18121971966418876No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA950.1756721772254891No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA930.17197381559968933No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA860.15902954990939014No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.15902954990939014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.14238692259329117No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA760.1405377417803913No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA740.13683938015459152No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT720.13314101852879176No Hit
TATCAACGCAGAGTGAATGGGTCAGCTATGCCA710.13129183771589187No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA700.12944265690299198No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA680.1257442952771922No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12389511446429231No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA650.12019675283849254No Hit
ACGCAGAGTGAATGGGTCAGCTATGCCAAAAAA610.11280002958689302No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT600.11095084877399312No Hit
CCCCTTCAGCTATGCCAACGTACTCTGCGTTGA600.11095084877399312No Hit
CCCGTCAGCTATGCCAACGTACTCTGCGTTGAT580.10725248714819335No Hit
GCTATGCCAAAAAAAAAAAAAAAAAAAAAAAAA550.10170494470949369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG200.00858500426.9703410
GCTAGGC200.00858500426.9703412
AACCCTC200.00858500426.970345
CTAGGCC200.00858500426.9703413
AGGCCAA200.00858500426.9703415
GGCCAAC200.00858500426.9703416
TAGGCCA200.00858500426.9703414
TATCTAC401.8270366E-523.5990491
GTATCAT300.002251294422.4752861
AACCTCA300.002251294422.4752865
TATCATC300.002251294422.4752862
ATCATCG300.002251294422.4752863
TCATCGC300.002251294422.4752864
CATCGCA300.002251294422.4752865
TCGCAGA300.00226347322.4544757
ATCGCAG300.00226347322.4544756
ATCTACG454.5373963E-520.9769332
TCTACGC454.5373963E-520.9769333
TTCTCAG454.5700057E-520.957516
CCTCTCA404.979392E-420.2277575
Produced by FastQC (version 0.11.2)