FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_62.35200000060248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_62.35200000060248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48377
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA14963.0923786096698844No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC5131.0604212745726274No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA3710.7668933584141224No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA3280.6780081443661244No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA2830.5849887343158939No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.5787874403125453No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA2610.5395125782913368No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA2260.46716414825226865No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA2220.45889575624780377No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.36794344419868946No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC1690.34933956218864337No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA1500.3100647001674349No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1480.30593050416520245No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA1460.30179630816297004No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA1450.2997292101618538No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA1410.29146081815738883No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1390.28732662215515636No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA1360.2811253281518077No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA1360.2811253281518077No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA1350.2790582301506914No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA1340.2769911321495752No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA1320.27285693614734274No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT1230.25425305413729665No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA1210.2501188581350642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.24805176013394792No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA1170.24185046613059924No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT1160.23978336812948303No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC1120.2315149761250181No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA1110.22944787812390188No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA1100.22738078012278562No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA1100.22738078012278562No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.2067098001116233No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.19430721210492588No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA930.19224011410380965No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA880.1819046240982285No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA870.17983752609711226No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA850.17570333009487982No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA820.1695020360915311No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA770.15916654608594993No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA760.1570994480848337No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCATA760.1570994480848337No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT750.15503235008371746No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA740.15296525208260123No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT720.14883105608036876No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCATAC720.14883105608036876No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA700.1446968600781363No Hit
ACAGAGCATACGTACTCAAAAAAAAAAAAAAAA690.14262976207702005No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT690.14262976207702005No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA690.14262976207702005No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA680.14056266407590384No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA670.1384955660747876No Hit
GAGTGAATGGGCAACAGAGCATACGTACTCAAA660.13642846807367137No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA660.13642846807367137No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA660.13642846807367137No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA650.13436137007255514No Hit
CAACTCAGAGTGAATGGGCAACAGAGCAAAAAA640.1322942720714389No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA630.13022717407032267No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC630.13022717407032267No Hit
GAGTGAATGGGGCAACAGAGCAAAAAAAAAAAA630.13022717407032267No Hit
AACGCAGAGTGAATGGGCAACAGAGCATAAAAA620.12816007606920643No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.12609297806809022No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA610.12609297806809022No Hit
CAACGCAGAGTGAATGGGCAACAGAGCAAAAAA590.12195878206585774No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCAAA590.12195878206585774No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA590.12195878206585774No Hit
GAGCATACGTACTAAAAAAAAAAAAAAAAAAAA580.11989168406474152No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA580.11989168406474152No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA580.11989168406474152No Hit
ACGCAGAGTGAATGGGGCAACAGAGCAAAAAAA570.11782458606362527No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA560.11575748806250905No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.11575748806250905No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA530.10955619405916034No Hit
CATACGTACTCAAAAAAAAAAAAAAAAAAAAAA530.10955619405916034No Hit
TATCAACGCAGAGTGAATGGGCAACAGAAAAAA520.10748909605804412No Hit
GTGAATGGGGCAACAGAGCATACGTACTCAAAA500.10335490005581165No Hit
CCTCGCAACAGAGCATACGTACTCTGCGTTGAT500.10335490005581165No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAA490.10128780205469541No Hit
GCATACGTACAAAAAAAAAAAAAAAAAAAAAAA490.10128780205469541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.4298653E-826.9885881
CAACTCA651.1641532E-1024.9125421
TACGCAG601.2714736E-924.739545
TCTACGC603.4804543E-822.4904923
TATCTAC603.4804543E-822.4904921
TATCATC553.779296E-722.0815732
ATCTACG658.1183316E-820.7604522
CATCGCA658.1183316E-820.7604525
ATCATCG608.726911E-720.2414423
TCGCAGA701.774024E-719.2775657
ATCGCAG701.774024E-719.2775656
TCATCGC651.8795326E-618.6844064
AACTCAG803.5084668E-818.5546552
ATACCAC753.5459743E-718.04856127
CGCTGAG601.988599E-517.9923932
GCTGAGT601.988599E-517.9923933
AACGTAG753.664518E-717.9923921
CTACGCA753.664518E-717.9923924
ACTCAGA857.021663E-817.4632033
CTCAGAG857.021663E-817.4632034
Produced by FastQC (version 0.11.2)