FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_61.3520000006022e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_61.3520000006022e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39972
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA4271.0682477734414089No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC2680.670469328529971No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA2610.6529570699489643No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA2440.6104272991093765No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC2320.5804062843990794No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA2150.5378765135594916No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA1920.48033623536475534No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1530.3827679375562894No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1420.3552486740718503No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1230.3077154007805464No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA1230.3077154007805464No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA980.24517162013409388No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.22765936155308714No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA910.22765936155308714No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA850.21264885419793855No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA840.21014710297208045No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC780.19513659561693183No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC760.19013309316521565No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.16761733213249275No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA650.16261382968077656No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA640.16011207845491846No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA630.15761032722906035No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA620.15510857600320224No Hit
ACACCATAGAGTCAAAAAAAAAAAAAAAAAAAA610.15260682477734414No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA580.14510157109976984No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA580.14510157109976984No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA580.14510157109976984No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT570.14259981987391174No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA560.14009806864805363No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA560.14009806864805363No Hit
GTACGTGACTCTAAAAAAAAAAAAAAAAAAAAA550.13759631742219555No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA550.13759631742219555No Hit
GAGTACGTGACTCTAAAAAAAAAAAAAAAAAAA540.13509456619633745No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA540.13509456619633745No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT530.13259281497047934No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA520.13009106374462123No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA510.12758931251876313No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA510.12758931251876313No Hit
GTGAATGGGGCACCATAGAGAAAAAAAAAAAAA480.12008405884118883No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA480.12008405884118883No Hit
GTCACGTACTCAAAAAAAAAAAAAAAAAAAAAA470.11758230761533073No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11758230761533073No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTA460.11508055638947262No Hit
ACACCATAGAGAAAAAAAAAAAAAAAAAAAAAA460.11508055638947262No Hit
GTGAATGGGGCACCAAAAAAAAAAAAAAAAAAA450.11257880516361454No Hit
ACTCTATGGTGTAAAAAAAAAAAAAAAAAAAAA450.11257880516361454No Hit
GTGAATGGGGAGTACGTGACTCTAAAAAAAAAA440.11007705393775644No Hit
ACTCTATGGTGAAAAAAAAAAAAAAAAAAAAAA430.10757530271189833No Hit
GAGTGAATGGGCACCATAGAGTAAAAAAAAAAA430.10757530271189833No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA420.10507355148604022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.6139806E-427.0489941
TGGTGAC200.00854184826.9812037
TATCATC352.0008984E-423.1267452
ATCATCG300.002235839422.4843373
ATCGCAG300.002235839422.4843376
TGGTGTG300.002235839422.4843377
TCATCGC300.002235839422.4843374
CTACGCA404.934671E-420.2359034
ATCTACG350.005463670519.2722872
TCTACGC350.005463670519.2722873
TCGCAGA350.005463670519.2722877
CATCGCA350.005463670519.2722875
GATACCA601.9367739E-518.03266326
TATCAAC2400.018.0326631
ATCAACG2550.016.9293822
ATACCAC653.8521663E-516.64553527
ATGGTGA757.3116953E-616.1887236
TGATACC1301.4733814E-1015.60518927
TACTCAA550.00416569514.71702114
TCAACGC3000.014.3899753
Produced by FastQC (version 0.11.2)